贵州医科大学学报

2004, (06) 488-491

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

丹芍化纤胶囊对肝纤维化大鼠肝组织结缔组织生长因子表达的影响
Effects of Danshaohuaxian Capsules on Expression of CTGF in Rat Hepatic Fibrosis

韩冰,杨勤,谢汝佳,罗新华,耿晓霞,程明亮
HAN Bing 1, YANG Qin 1, XIE Ru jia 1, LUO Xin hua 2, GENG Xiao xia 3, CHENG Ming liang 3 (1.Department of Pathophysiology, Guiyang Medical College, Guiyang 550004, China; 2. Department of Infectious Diseases, People’s Hospital of Guizhou Province, Guiyang 550001, China; 3.Department of Infectious Diseases, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China)

摘要(Abstract):

目的 :研究结缔组织生长因子 (CTGF)在丹芍化纤胶囊治疗大鼠实验性肝纤维化组织中的表达 ,探讨丹芍化纤胶囊延缓肝脏纤维化的药理机制。方法 :雄性Wistar大鼠 80只分为正常组、肝纤维化模型组、自然恢复组、低剂量治疗组、高剂量治疗组 ,除正常组外 ,采用四氯化碳 (CCl4)、饮酒、高脂低蛋白饮食等复合病因制备肝纤维化动物模型 ,低剂量组和高剂量组分别给予 0 5g/kg、1g/kg丹芍化纤胶囊灌胃治疗 8周 ,自然恢复组则给予等容量生理盐水。实验结束后测定血清透明质酸 (HA)及谷丙转氨酶 (ALT) ,光镜下观察肝组织纤维化程度 ,同时用免疫组织化学法 (SABC)检测肝组织中CTGF的表达。结果 :与肝纤维化模型组、自然恢复组比较 ,治疗组血清HA及ALT显著下降 ,肝纤维化程度明显减轻 ,肝组织中CTGF的表达明显减少。结论 :丹芍化纤胶囊能够抑制肝纤维化组织中CTGF的表达 ,这可能是丹芍化纤胶囊抗肝纤维化作用的机制之一。
Objective: To investigate the effects of Danshaohuaxian (DSHX) capsule, a traditional Chinese medicine prescription,on the expression of connective tissue growth factor (CTGF) in rat with hepatic fibrosis, so as to explore the mechanism by which DSHX delays the hepatic fibrosis progression. Methods: Eighty male Wistar rats were randomly divided into normal control group (NC), CCl 4 induced hepatic fibrosis group (HF), non DSHX treated group (NT), low dose treated group (LD) and high dose treated group (HD). Except for the normal control group, the rat hepatic fibrosis were induced by subcutaneous injection of CCl 4, drinking alcohol, diet of hyperliposis and hypoproteinia for 8 weeks. When hepatic fibrosis models were produced, the rats in groups of LD and HD were treated with DSHX capsules at low dose (0 5 g/kg) and high dose (1 0 g/kg) respectively by p.o everyday for 8 weeks. At the end of the experiment, the serum hyaluronic acid (HA) and alanine aminotransferase (ALT) were examied; the hepatic fibrosis degree was evaluated with optical microscopy; the expression of CTGF was detected with immunohistochemical techniques. Results: Compared with all the indexes of HF and NT groups, it was revealed that serum HA and ALT were significantly reduced and the degree of hepatic fibrosis was abated in the two treated groups; the expression of CTGF was decreased obviously. All these alterations in HD group were more obvious than that in LD group. Conclusions: The DSHX capsule could inhibit the expression of CTGF in liver tissues of CCl 4 induced hepatic fibrosis rats, which might be one of its anti hepatic fibrosis mechanisms. [

关键词(KeyWords): 肝硬化;丹芍化纤胶囊;结缔组织;生长物质;大鼠
liver cirrhosis; Danshaohuaxian capsule; connective tissue; growth substances; rats

Abstract:

Keywords:

基金项目(Foundation): 贵阳医学院科研基金资助项目

作者(Author): 韩冰,杨勤,谢汝佳,罗新华,耿晓霞,程明亮
HAN Bing 1, YANG Qin 1, XIE Ru jia 1, LUO Xin hua 2, GENG Xiao xia 3, CHENG Ming liang 3 (1.Department of Pathophysiology, Guiyang Medical College, Guiyang 550004, China; 2. Department of Infectious Diseases, People’s Hospital of Guizhou Province, Guiyang 550001, China; 3.Department of Infectious Diseases, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China)

Email:

DOI: 10.19367/j.cnki.1000-2707.2004.06.007

文章评论(Comment):

序号(No.) 时间(Time) 反馈人(User) 邮箱(Email) 标题(Title) 内容(Content)
反馈人(User) 邮箱地址(Email)
反馈标题(Title)
反馈内容(Content)
扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享