王荣新;雷大卫;苏晓庆;
WANG Rongxin1, LEI Dawei2, SU Xiaoqing1(1.Department of Biology, Guiyang Medical College, Guiyang 550004, Guizhou, China; 2.Department of Biochemistry, Guiyang Medical College, Guiyang 550004, Guizhou, China)

• 1:贵阳医学院生物学教研室
• 2:贵阳医学院生物化学教研室
• 3:贵阳医学院生物学教研室 贵州贵阳550004
• 4:贵州贵阳550004

摘要(Abstract)：

目的:了解不同诱导条件对贵阳腐霉(Pythium guiyangense Su)产生Pr1酶的影响。方法:观察了不同诱导物、不同菌丝接种量、不同初始pH对贵阳腐霉产生Pr1酶的影响。结果:发现在无诱导物培养液中,无Pr1酶活性。以虾壳为诱导物,在1%菌丝接种量时,产生的Pr1酶活力较高,初始pH对贵阳腐霉产生Pr1蛋白酶的影响不大。结论:了解了贵阳腐霉产生Pr1酶的最适条件,为以后研究贵阳腐霉提供参考。
Objective: To investigate the effects of different induction conditions on subtilisin-like protease(Pr1)production of P. guiyangense. Methods: Effects of different inducers, inoculated hyphal quantity and initial pH on Pr1 production of P. guiyangense were observed. Results: No Pr1 activity (A410 values) in media was found when no inducer was used. The enzyme activity of Pr1 produced by P.guiyangense was relatively high under the conditions that using shrimp cuticle as inducer,and hyphal inoculation quantity being 1g in 100 ml of culture media. Initial pH didn’t show remarkable effect on Pr1 production. Conclusions: A relatively optimized protocol for induction of Pr1 from P. guiyangense has been summarized, which is useful for future research work.

关键词(KeyWords)： 真菌;灭蚊;蛋白质类;酶诱导;贵阳腐霉;Pr1蛋白酶
fungi; mosquito control; proteins; enzyme induction; Pythium guiyangensesu Su; subtilisin-like proteases

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(批准号:30160003);; 贵州省国际科技合作重点项目[合同号:黔科合外字(2005)400118号]

作者(Author): 王荣新;雷大卫;苏晓庆;
WANG Rongxin1, LEI Dawei2, SU Xiaoqing1(1.Department of Biology, Guiyang Medical College, Guiyang 550004, Guizhou, China; 2.Department of Biochemistry, Guiyang Medical College, Guiyang 550004, Guizhou, China)

Email:

DOI: 10.19367/j.cnki.1000-2707.2008.01.006

参考文献(References)：

• [1]徐均焕,冯明光,许谦,等.昆虫病原真菌胞外蛋白酶的病理作用与测定技术[J].中国生物防治,1998(3):123-128.
• [2]王滨,李增智,樊美珍,等.昆虫病原真菌基因研究进展[J].菌物系统,2001(3):430-434.
• [3]苏晓庆.大链壶菌和卡地腐霉的Prl和Pr2类蛋白酶的研究[J].贵阳医学院学报,2002(5):377-380.
• [4]姚剑,李增智,樊美珍.虫生真菌入侵过程中酶的研究[J].安徽农业大学学报,1996(3):303-308.
• [5]杨星勇,王中康,夏玉先,等.球孢白僵菌凝乳弹性蛋白酶(BbPr1)的纯化与特性[J].菌物系统,2000(2):254-256.
• [6]裴炎,冀志霞,杨星勇,等.绿僵菌分解昆虫外壳蛋白酶MAP-21的纯化与特性[J].微生物学报,2000(3):306-311.
• [7]张洁,蔡敬民,吴克,等.球孢白僵菌Bb174固态发酵产几丁质酶产酶及酶学特征研究[J].应用生态学报,2004(5):863-866.
• [8]蔡荟梅,刘斌,蔡敬民,等.绿僵菌Ma83固态发酵几丁质酶和部分酶学性质的初步研究[J].食品与发酵工业,2003(2):152-156.
• [9]蒲蛰龙,李增智.昆虫真菌学[M].合肥:安徽科学技术出版社,1996:69-75.
• [10]张永军,彭国雄,方卫国,等.球孢白僵菌胞外蛋白酶及类枯草杆菌蛋白酶的诱导[J].应用与环境生物学报,2000(2):182-186.
• [11]顾天爵,冯宗枕.生物化学[M].4版.北京:人民卫生出版社,1995:43.

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