贵州医科大学学报

2008, No.130(01) 12-15

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贵阳腐霉Pr1蛋白酶的诱导条件研究
An Observation on the Induction Conditions for the Subtilisin-like Protease Prodcution from Pythium guiyangense Su, A Mosquito-killing Fungus

王荣新;雷大卫;苏晓庆;
WANG Rongxin1, LEI Dawei2, SU Xiaoqing1(1.Department of Biology, Guiyang Medical College, Guiyang 550004, Guizhou, China; 2.Department of Biochemistry, Guiyang Medical College, Guiyang 550004, Guizhou, China)

摘要(Abstract):

目的:了解不同诱导条件对贵阳腐霉(Pythium guiyangense Su)产生Pr1酶的影响。方法:观察了不同诱导物、不同菌丝接种量、不同初始pH对贵阳腐霉产生Pr1酶的影响。结果:发现在无诱导物培养液中,无Pr1酶活性。以虾壳为诱导物,在1%菌丝接种量时,产生的Pr1酶活力较高,初始pH对贵阳腐霉产生Pr1蛋白酶的影响不大。结论:了解了贵阳腐霉产生Pr1酶的最适条件,为以后研究贵阳腐霉提供参考。
Objective: To investigate the effects of different induction conditions on subtilisin-like protease(Pr1)production of P. guiyangense. Methods: Effects of different inducers, inoculated hyphal quantity and initial pH on Pr1 production of P. guiyangense were observed. Results: No Pr1 activity (A410 values) in media was found when no inducer was used. The enzyme activity of Pr1 produced by P.guiyangense was relatively high under the conditions that using shrimp cuticle as inducer,and hyphal inoculation quantity being 1g in 100 ml of culture media. Initial pH didn’t show remarkable effect on Pr1 production. Conclusions: A relatively optimized protocol for induction of Pr1 from P. guiyangense has been summarized, which is useful for future research work.

关键词(KeyWords): 真菌;灭蚊;蛋白质类;酶诱导;贵阳腐霉;Pr1蛋白酶
fungi; mosquito control; proteins; enzyme induction; Pythium guiyangensesu Su; subtilisin-like proteases

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(批准号:30160003);; 贵州省国际科技合作重点项目[合同号:黔科合外字(2005)400118号]

作者(Author): 王荣新;雷大卫;苏晓庆;
WANG Rongxin1, LEI Dawei2, SU Xiaoqing1(1.Department of Biology, Guiyang Medical College, Guiyang 550004, Guizhou, China; 2.Department of Biochemistry, Guiyang Medical College, Guiyang 550004, Guizhou, China)

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DOI: 10.19367/j.cnki.1000-2707.2008.01.006

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