贵州医科大学学报

2009, v.34;No.140(05) 504-507

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整体低氧预处理对大鼠肺组织细胞色素C表达和细胞凋亡的影响
The Influence of Whole-body Hypoxic Preconditioning on Cytochrome C Expression and Apoptoss in Rats with Ischemia/Peperfusion Caused Lung Injury

陈莹;陆德琴;李涛;李保罗;
CHEN Ying1, LU Deqin1, LI Tao2, LI Baoluo1(Department of Pathophysiology,Guiyang Medical College,Guiyang 550004,Guizhou China,)

摘要(Abstract):

目的:探讨整体低氧预处理(HPC)对肺缺血-再灌注损伤(I/R)的保护效应。方法:重复低氧5次,建立大鼠HPC模型,用无创微血管夹夹闭左侧肺门45min,1h后松开再灌注180min建立肺I/R模型;设立假手术组、肺I/R组、HPC+肺I/R组,HE染色观察各组肺组织病理学改变,称重计算各组肺湿重与干重比值,免疫组织化学染色方法检测肺组织内细胞色素C的表达,TUNEL法检测肺细胞凋亡。结果:与假手术组相比,肺I/R组肺组织病理学改变明显,肺组织湿/干重比、肺组织细胞色素C表达及凋亡指数显著增高(P<0.05);与肺I/R组比较,HPC+肺I/R组肺组织病理学改变明显改善,其余各检测指标均降低(P<0.05)。结论:大鼠整体低氧预处理可减少肺组织细胞线粒体释放细胞色素C,抑制细胞凋亡从而保护肺I/R损伤。
Objective:To investigate the protective effect of whole-body hypoxic preconditioning (HPC) on ischemia/reperfusion(lung-I/R)caused lung injury in rats. Methods: HPC model rat was established with repetitive sublethal hypoxia for 5 times,while lung-I/R rat was prepared with left lung hilar clamping for 45 min followed by 180-min reperfusion. Thirty rats were randomly divided into 3 groups (n=10,respectively): sham-operation group (group S),lung-I/R group (group I/R) and HPC+lung-I/R group (group H). After the experiment,lung weights were measured and wet/dry ratioes were calculated,lung morphological changes were observed on HE stained sections,expression of cytochrome C was detected with immunohistochemitry method,and cell apoptosis was assessed with TUNEL method. Result: Compared with those in group S,pathological changes of lung tissue were more obvious,and wet/dry ratio,cytochrome C expression,and cell apoptosis index were significantly increased(P<0.05)in group I/R;While in group H,lung tissue pathological chages were milder,and the other indexes were markedly lowered than those in group I/R. Conclusion: Whole-body hypoxic preconditioning in rats can protect lung from I/R caused injury by reducing release of cytochrome C and inhibiting cell apoptosis.

关键词(KeyWords): 肺;低氧血症;缺血;再灌注损伤;细胞色素C细胞凋亡;大鼠,Spague-Dawley
lung;anoxemia;ischemia;reperfusion injury;cytochrosome C;apoptosis;rats,sprague-dawley

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基金项目(Foundation): 贵州省省长基金资助项目(S2003-9)

作者(Author): 陈莹;陆德琴;李涛;李保罗;
CHEN Ying1, LU Deqin1, LI Tao2, LI Baoluo1(Department of Pathophysiology,Guiyang Medical College,Guiyang 550004,Guizhou China,)

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DOI: 10.19367/j.cnki.1000-2707.2009.05.008

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