贵州医科大学学报

1992, (03) 227-229

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用四氮唑蓝法测定大鼠肝、脑组织中Mn—SOD的活力
The Activity of Mn-SOD of the Liver and Brain of Rats Determined by Tetrazolium Blue Assay

卢步峰;张小蕾;唐筑灵;
Lu Bufeng, et al Zhenjiang Medical College

摘要(Abstract):

本文改进了四氮唑蓝法,对大鼠肝、大脑皮层、小脑和脑干中的锰-超氧化物歧化酶(Mn-SOD)活力进行测定。该法优点是:简便、灵敏度高、重复性好(变异系数C.V=4.2%),对仪器和试剂要求低,不需要SOD标准品.
An adapted tetrazolium blue assay in determining the activity of Mn superoxide dismutase (Mn-SOD) in tissues was introduced. The method was easy operating, highly sensitive and accurate (C.V=4.2%).Using this method, we determined the Mn-SOD activities of the adult rat liver, cerebral cortex, cerebellum and brain stem (n=13). The results were 5.776±1.496, 4.422±1.315, 7.226±2.341 and 7.065±2.667 U/mg Pr respectively. Some affecting factors in procedure of the assay were discussed.

关键词(KeyWords): 超氧化物歧化酶类;肝脑;大鼠;四氮唑蓝法
superoxide dismutase;liver;brain;rats;tetrazolium blue assay

Abstract:

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作者(Author): 卢步峰;张小蕾;唐筑灵;
Lu Bufeng, et al Zhenjiang Medical College

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DOI: 10.19367/j.cnki.1000-2707.1992.03.018

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