卢步峰;张小蕾;唐筑灵;
Lu Bufeng, et al Zhenjiang Medical College

• 1: 镇江医学院
• 2:贵州省老年医学研究所
• 3:贵州省老年医学研究所

摘要(Abstract)：

本文改进了四氮唑蓝法,对大鼠肝、大脑皮层、小脑和脑干中的锰-超氧化物歧化酶(Mn-SOD)活力进行测定。该法优点是:简便、灵敏度高、重复性好(变异系数C.V=4.2％),对仪器和试剂要求低,不需要SOD标准品.
An adapted tetrazolium blue assay in determining the activity of Mn superoxide dismutase (Mn-SOD) in tissues was introduced. The method was easy operating, highly sensitive and accurate (C.V=4.2%).Using this method, we determined the Mn-SOD activities of the adult rat liver, cerebral cortex, cerebellum and brain stem (n=13). The results were 5.776±1.496, 4.422±1.315, 7.226±2.341 and 7.065±2.667 U/mg Pr respectively. Some affecting factors in procedure of the assay were discussed.

关键词(KeyWords)： 超氧化物歧化酶类;肝脑;大鼠;四氮唑蓝法
superoxide dismutase;liver;brain;rats;tetrazolium blue assay

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 卢步峰;张小蕾;唐筑灵;
Lu Bufeng, et al Zhenjiang Medical College

Email:

DOI: 10.19367/j.cnki.1000-2707.1992.03.018

参考文献(References)：

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• 2 黄维嘉,等.邻苯三酚自氧化抑制法测定人红细胞SOD.中华医学检验杂志,1989,12(4) :206
• 3 李益新.超氧化物歧化酶活力测定的 新方法--化学发光法.生物化学与生物物理进展,1983,10(2) :59
• 4 许平.超氧化物歧化酶活力测定.中华医学检验杂志,1987,10(4) :209
• 5 汪义军,等.血清锰和铜锌超氧化物歧化酶活力测定。生物化学与生物物理进展,1990,17(3) :237

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