贵州医科大学学报

2009, v.34;No.140(05) 497-500

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梯度洗脱反相HPLC法测定刺薏胶囊中紫丁香苷含量
Determination of Syringoside in Ciyi Capsule with Gradient Elution Reverse Phase HPLC

麻秀萍;蒋朝晖;贾宪生;丁宁;叶世云;吴晓黎;
MA Xiuping,JIANG Zhaohui,JIA Xiansheng,DING Ning,YE Shiyun,WU Xiaoli(Department of Phamacology,Guiyang College of Traditional Chinese Medicine,Guiyang 550002,Guizhou,China)

摘要(Abstract):

目的:建立测定刺薏胶囊中紫丁香苷含量的高效液相色谱法,并进行方法学考察。方法:采用梯度洗脱法,用ZORBAXSB-C18(5μm,4.6mm×150mm)色谱柱,以甲醇为流动相A,水为流动相B,以1.0ml/min的流速进行梯度洗脱(0~20min,A为18%;20~30min,A为18%~70%;30~35min,A从70%~18%);检测波长265nm。结果:紫丁香苷进样量在0.0808~0.404μg与峰面积呈良好的线性关系,r=0.9999;平均回收率为98.2%,RSD为1.75%。结论:梯度洗脱反相HPLC法测定刺薏胶囊中紫丁香苷含量的方法专属性强、准确稳定、重复性好,可用于刺薏胶囊的质量监控。
Objective:To establish a method for the determination of syringoside in Ciyi compound preparation with reverse phase high pressure liquid chromatography (RP-HPLC) and to evaluate the methodology. Methods: The column was ZORBAX SB-C18(5 μm,4.6 mm× 150 mm ).The flow rate was 1.0 ml/min. The mobile phase A was methanol and mobile phase B water. The procedures were that: at ranges of 0~20 min,20~30,and 30~35 min,concentrations of A were 18%,18%~70%,and 70%~18% respectively. The detection wavelength was 265 nm. Results: When the sample sizes were in the range of 0.080 8~0.404 μg,the standard curve for syringoside was satisfactorily linear (r=0.999 9). The average recovery was 98.2% with RSD=1.75% (n=6). Conclusions: The method is specific,accurate,and stable,and it can be used for detemination of syringoside in Ciyi compound preparation.

关键词(KeyWords): 刺薏胶囊;紫丁香苷;色谱法,高压液相
Ciyi capsule;syringin;chromatography,high pressure liquid

Abstract:

Keywords:

基金项目(Foundation): 贵州省中药现代化专项科技项目[黔科合中药专字(2003)73号]

作者(Author): 麻秀萍;蒋朝晖;贾宪生;丁宁;叶世云;吴晓黎;
MA Xiuping,JIANG Zhaohui,JIA Xiansheng,DING Ning,YE Shiyun,WU Xiaoli(Department of Phamacology,Guiyang College of Traditional Chinese Medicine,Guiyang 550002,Guizhou,China)

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DOI: 10.19367/j.cnki.1000-2707.2009.05.006

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