贵州医科大学学报

1997, (03)

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斑点-酶联免疫吸附试验检测卡氏肺孢子虫
The Diagnosis of Pneumocystis Carinii Pneumonia by Dot ELISA

陈艳,万明明,王宝林,毛克强
Chen Yan,et al Departmant of Parasitology,Guiyang Medical College

摘要(Abstract):

应用完整的卡氏肺孢子虫包囊作抗原,以4×107/ml包囊点样,作斑点-酶联免疫吸附试验(Dot-ELISA)检测肺印片卡氏肺孢子虫包囊阳性的BALB/c小鼠血清20份,同时检测包囊阴性的小鼠血清20份,结果除一份小鼠血清IgG阴性反应外,均为阳性反应,阳性率为95%;而正常对照血清全为阴性反应。另外,检测了3份纤支镜刷检物中包囊阳性的病人血清,均为阳性反应;50例健康孕妇血清的检测,抗体阳性率为12%(6/50)。
Twenty wistar rats were injected with cortisone acetate twice a week subcutaneously for 8 10 weeks.The cysts of Pneumocystis Carinii from infected lungs of cortisonied rats were indentified by Giensa’s stain.The lung tissue was collected.The discontinuous percoll density gradient centrifugation was used to separate P.carinii cysts.The whole cysts (4×10 7/ml) were used as antigens in Dot ELISA.The specifice IgG antibodies in the sera from 20 infected BALB/c mice were detected by Dot ELISA.Only one of 20 sera showed negation reaction,while all 20 sera from normal BALB/c mice that their lung tissues had not P.carinii cysts gave negative rection.3 serum samples from the patients with P.carinii pneumonia were positive rection.The 50 normal individuals were detected.The total positive rate was 12% (6/50).

关键词(KeyWords): 卡氏肺孢子虫,斑点-酶联免疫吸附试验
Pneumocystis Carinii;Dot ELISA

Abstract:

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基金项目(Foundation): 贵州省卫生厅资助

作者(Author): 陈艳,万明明,王宝林,毛克强
Chen Yan,et al Departmant of Parasitology,Guiyang Medical College

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DOI: 10.19367/j.cnki.1000-2707.1997.03.011

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