贵州医科大学学报

2013, (01) 19-23

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大鼠肝纤维化过程中miR-141表达的变化
Changes of miR-141 Expression during Development of Rat Liver Fibrosis

洪琴;张成俊;谢汝佳;韩冰;杨婷;杨勤;
HONG Qin,ZHANG Chengjun,XIE Rujia,HAN Bing,YANG Ting,YANG Qin(Department of Pathophysiology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

摘要(Abstract):

目的:探讨微小RNA-200家族成员中微小RNA-141(miR-141)在大鼠肝纤维化过程的表达变化及其作用。方法:雄性Wistar大鼠32只,随机分为正常对照组及肝纤维化组。皮下注射60%CCl4制备肝纤维化模型,分别在造模4周、8周末处死大鼠,测定肝脏指数、血清谷丙转氨酶(ALT)和谷草转氨酶(AST)的含量并观察肝组织结构改变。实时荧光定量PCR方法测定各组大鼠肝组织中miR-141的表达水平。结果:造模不同时间肝纤维化组大鼠肝脏指数、血清ALT和AST活性均显著高于相应正常对照组(P<0.01)。病理学观察发现造模4周大鼠肝脏纤维结缔组织明显增多,且随时间增长纤维化病变逐步加重,在肝纤维化8周组大鼠肝脏中有明显假小叶形成。Real time-PCR结果显示,大鼠肝脏miR-141水平肝纤维化4周组较相应对照组明显降低(P<0.01),肝纤维化8周组明显高于相应正常对照组(P<0.01)及肝纤维化4周组(P<0.01)。结论:研究表明CCl4诱导大鼠肝脏miR-141的表达水平有明显的改变,提示肝脏miR-141可能通过某种机制参与了大鼠肝纤维化的发生发展。
Objective: To investigate the expression changes and function of microRNA-141(miR-141),one member of the microRNA-200 family,in the process of hepatic fibrosis in rats.Methods: Thirty-two male Wistar rats were randomly divided into normal control groups and liver fibrosis groups.Liver fibrosis model was established by hypodermic injection of 60% CCl4.The rats were sacrificed in 4 and 8 weeks respectively,and experimental groups were named liver fibrosis groups 4-week and 8-week.Liver index,the contents of glutamic propylic transaminase(ALT) and glutamic oxaolacetic transaminase(AST) were detected,and morphological changes of liver were observed.Real-time fluorescence quantitative PCR was employed to determine the expression levels of miR-141 in liver tissue of rats in each group.Results: Liver index,serum ALT and AST levels in each liver fibrosis group were obviously higher than those in control groups(P<0.01).Pathological discovery showed that fibrous connective tissue significantly increased in 4 weeks after liver fibrosis model established and this change was more obvious with time passing,and false lobes were found in rat liver in group 8-week.The expression level of miR-141 in group 4-week was obviously lower than that in corresponding control group(P<0.01),and was higher in group 8-week than that in its corresponding control group(P<0.01) and that in group 4-week(P<0.01).Conclusions: Expression level of miR-141 obviously changes in the process of CCl4 induced liver fibrosis,Which suggests that miR-141 may be involved in the development of rat liver fibrosis.

关键词(KeyWords): 微小RNA;肝;纤维化;大鼠,Wistar
microRNA;liver;fibrosis;rats,Wistar

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基金项目(Foundation): 贵阳医学院研究生教育创新计划专项经费资助,课题编号:合同字第S201110号;; 贵州省省长专项资金项目,课题编号:黔省专合字(2010)41号;; 贵州省科技厅资助项目,课题编号:2011GZ39836

作者(Author): 洪琴;张成俊;谢汝佳;韩冰;杨婷;杨勤;
HONG Qin,ZHANG Chengjun,XIE Rujia,HAN Bing,YANG Ting,YANG Qin(Department of Pathophysiology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

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