贵州医科大学学报

1989, (02) 111-115+161

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

L型细菌培养特性的观察
Observation on Characteristics of Culture of L Forms

王和;
Wang He Department of Microbiology

摘要(Abstract):

本文将金黄色葡萄球菌L型,表皮葡萄球菌L型和铜绿假单胞菌L型接种非高渗透性的和高渗透性的1640液、Eagle,s液及营养肉汤中传代培养和接种单层HeLa细胞、L型培养基及软琼脂平板等培养基中培养。结果表明葡萄球菌L型和铜绿假单胞菌L型可在非高渗透性的和高渗透性的液体培养基中传代培养,并可致HeLa细胞病变。但未能在固体或软琼脂平板培养基中形成生长现象。
L forms of staphylococcus aureus, staphylocccous epidermidis and pseudomonas aeruginosa were subcultured in non-high osmotic and high osmotic liquid media of RPMI, Eagle' sand nutritions brot h and cultured inmonolayer cultures of He Lacells, L form media and soft agar plate media and so on. The results show that L forms of staphylococcus spp. and pseudomonasaeruginosa can be subcul tured in the non-high osmotic and high osmotic liquid media and cause cyto- pathy in HeLa cells, but the growth of L forms in solid or soft agar plate me- dia can not be seen.

关键词(KeyWords): L型菌;渗透性;培养基;细菌学技术
L forms; osmosis; cultue media; bacteriological mechnics

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 王和;
Wang He Department of Microbiology

Email:

DOI: 10.19367/j.cnki.1000-2707.1989.02.010

文章评论(Comment):

序号(No.) 时间(Time) 反馈人(User) 邮箱(Email) 标题(Title) 内容(Content)
反馈人(User) 邮箱地址(Email)
反馈标题(Title)
反馈内容(Content)
扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享