贵州医科大学学报

2008, No.131(02) 173-175

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调经活血胶囊芍药苷含量测定方法研究
Determination of Peoniflorin in Tiaojinghuoxue Capsules

罗忠圣;杨青松;吴永红;覃容贵;
LUO Zhongsheng1,YANG Qingsong1,WU Yonghong1,QIN Ronggui2(1 Guiyang Xintian Pharmaceutical Limited Company,Guiyang 550018,Guizhou,China;2.School of Pharmacology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

摘要(Abstract):

目的:建立调经活血胶囊中芍药苷的高效液相色谱法。方法:采用大连依利特C-18柱,甲醇-水(25:75)为流动相,检测波长为230nm,流速为1.0ml/min,柱温为25℃,检测调经活血胶囊芍药苷含量。结果:芍药苷的线性范围为10~100mg/L,平均回收率为99.6%,RSD为0.14%。结论:采用高效液相色谱法测定调经活血胶囊中芍药苷的含量,具有简便,快速,准确的优点。
Objective:To establish an HPLC method for determination of paeoniflorin in Tiaojinghuoxue Capsules.Methods:Elite Hypersil C18 column was used with methanol-water(25∶75)as mobile phase.The flow rate was 1.0ml/min,and the detection wave was 230nm and the column temperature was 25 ℃.Results:The linear range of paeoniflorin was 10~100 mg/L.The average recovery was 99.6 % and the RSD was 0.14 %.Conclusion:The method can be used for the determination of Paeoniflorin in Tiaojinghuoxue Capsules.

关键词(KeyWords): 调经活血胶囊;芍药甙;色谱法,高压液相
Tiaojinghuoxue Capsules;paeoniflorin;chromatography,high pressure liquid

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作者(Author): 罗忠圣;杨青松;吴永红;覃容贵;
LUO Zhongsheng1,YANG Qingsong1,WU Yonghong1,QIN Ronggui2(1 Guiyang Xintian Pharmaceutical Limited Company,Guiyang 550018,Guizhou,China;2.School of Pharmacology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

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DOI: 10.19367/j.cnki.1000-2707.2008.02.019

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