罗忠圣;杨青松;吴永红;覃容贵;
LUO Zhongsheng1,YANG Qingsong1,WU Yonghong1,QIN Ronggui2(1 Guiyang Xintian Pharmaceutical Limited Company,Guiyang 550018,Guizhou,China;2.School of Pharmacology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

• 1:贵阳新天药业股份有限公司
• 2:贵阳新天药业股份有限公司
• 3:贵阳新天药业股份有限公司
• 4:贵阳医学院药学院 贵州贵阳550018

摘要(Abstract)：

目的:建立调经活血胶囊中芍药苷的高效液相色谱法。方法:采用大连依利特C-18柱,甲醇-水(25:75)为流动相,检测波长为230nm,流速为1.0ml/min,柱温为25℃,检测调经活血胶囊芍药苷含量。结果:芍药苷的线性范围为10～100mg/L,平均回收率为99.6%,RSD为0.14%。结论:采用高效液相色谱法测定调经活血胶囊中芍药苷的含量,具有简便,快速,准确的优点。
Objective:To establish an HPLC method for determination of paeoniflorin in Tiaojinghuoxue Capsules.Methods:Elite Hypersil C18 column was used with methanol-water(25∶75)as mobile phase.The flow rate was 1.0ml/min,and the detection wave was 230nm and the column temperature was 25 ℃.Results:The linear range of paeoniflorin was 10～100 mg/L.The average recovery was 99.6 % and the RSD was 0.14 %.Conclusion:The method can be used for the determination of Paeoniflorin in Tiaojinghuoxue Capsules.

关键词(KeyWords)： 调经活血胶囊;芍药甙;色谱法,高压液相
Tiaojinghuoxue Capsules;paeoniflorin;chromatography,high pressure liquid

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 罗忠圣;杨青松;吴永红;覃容贵;
LUO Zhongsheng1,YANG Qingsong1,WU Yonghong1,QIN Ronggui2(1 Guiyang Xintian Pharmaceutical Limited Company,Guiyang 550018,Guizhou,China;2.School of Pharmacology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

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DOI: 10.19367/j.cnki.1000-2707.2008.02.019

参考文献(References)：

• [1]中华人民共和国卫生部部颁标准.WS3-B-0614-91调经活血片质量标准[S].1991:150.
• [2]国家药典委员会.中华人民共和国药典(一部)[S].北京:化学工业出版社,2005:109.
• [3]朱晓军,陈彬,方强,等.高效液相色谱法测定精制冠心颗粒中芍药苷含量[J].医药导报,2006(8):826-827.
• [4]林伟忠.白芍及其复方制剂中芍药苷测定方法研究[J].中成药,1997(6):43-44.
• [5]李矗,王汀,王珍.高效液相色谱法测定赤勺中赤勺总苷的含量[J].安徽医药,2005(11):820-821.
• [6]赵永德,丁昌成,贾善学.RP-HPLC法测定胃肠舒片中芍药苷的含量[J].中国药师,2005(12):1007-1008.

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