王荣新;雷大卫;苏晓庆;
WANG Rongxin1,LEI Dawei2,SU Xiaoqing1(1.Department of Biology,Guiyang Medical College,Guiyang 550004,China;2.Department of Biochemistry,Guiyang Medical College,Guiyang 550004,China)

• 1:贵阳医学院生物学教研室
• 2:贵阳医学院生物化学教研室
• 3:贵阳医学院生物学教研室 贵州贵阳550004
• 4:贵州贵阳550004

摘要(Abstract)：

目的:了解不同诱导条件对贵阳腐霉(Pythium guiyangense Su)产生昆虫表皮降解酶的影响。方法:观察不同诱导物、不同菌丝接种量,不同初始pH时,培养基中蛋白质浓度。结果:以蝉蜕为诱导物,在1%菌丝接种量,初始pH10.0的条件下时,培养基中蛋白质浓度较高。结论:本研究结果可反映贵阳腐霉产生昆虫表皮降解酶的条件,并可为酶的进一步分离纯化以及深入了解真菌的致病机理打下基础。
Objective: To study effects of different induction conditions on production of insect cuticle degrading protease from Pythium guiyangense Su.Methods: The protein concentration in culture media was observed after the fungus was induced under different conditions,including different inducer,hyphal quantity and initial pH.Results: The protein concentration in culture media was higher under the condition combination: Cicada exuviae as inducer,1% hyphal inoculation quantity and initial pH10.0.Conclusions: Results of this research could reflect production condition on insect cuticle degrading protease from P.guiyangense,lay the foundation for further isolation and purification of protease and to explore the disease-causing mechanism of P.guiyangense.

关键词(KeyWords)： 真菌;灭蚊;蛋白质类;酶诱导;贵阳腐霉;胞外蛋白质量
fungi;mosquito control;proteins;enzyme induction;Pythium guiyangense;the extracellular protein quantity

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(批准号30160003);; 贵州省省长基金和贵州省高层次人才特助经费资助

作者(Author): 王荣新;雷大卫;苏晓庆;
WANG Rongxin1,LEI Dawei2,SU Xiaoqing1(1.Department of Biology,Guiyang Medical College,Guiyang 550004,China;2.Department of Biochemistry,Guiyang Medical College,Guiyang 550004,China)

Email:

DOI: 10.19367/j.cnki.1000-2707.2007.05.003

参考文献(References)：

• [1]徐均焕,冯明光,许谦.昆虫病原真菌胞外蛋白酶的病理作用与测定技术[J].中国生物防治,1998(3):123-128.
• [2]顾天爵,冯宗枕.生物化学[M].4版.北京:人民卫生出版社,1995:43.
• [3]姚剑,李增智,樊美珍.虫生真菌入侵过程中酶的研究[J].安徽农业大学学报,1996(3):303-308.
• [4]杨星勇,王中康,夏玉先,等.球孢白僵菌凝乳弹性蛋白酶(BbPr1)的纯化与特性[J].菌物系统,2000(2):254-260.
• [5]裴炎,冀志霞,杨星勇,等.绿僵菌分解昆虫外壳蛋白酶MAP-21的纯化与特性[J].微生物学报,2000(3):306-311.
• [6]张洁,蔡敬民,吴克,等.球孢白僵菌Bb174固态发酵产几丁质酶产酶及酶学特征研究[J].应用生态学报,2004(5):863-866.
• [7]蔡荟梅,刘斌,蔡敬民,等.绿僵菌M a83固态发酵几丁质酶和部分酶学性质的初步研究[J].食品与发酵工业,2003(2):152-156.

文章评论(Comment)：