贵州医科大学学报

2009, v.34;No.136(01) 16-20

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糖尿病大鼠肾脏病变及其SnoN蛋白表达的变化
The Renal Lesions and SnoN Protein Expression in Kidney Tissue of Rats with Type 2 Diabetes

崔龙;刘瑞霞;李晓颖;石明隽;肖瑛;郭兵;张国忠;
CUI Long,LIU Ruixia,LI Xiaoying,SHI Mingjun,XIAO Ying,Guo Bing,ZHANG Guozhong(Department of Pathophysiology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

摘要(Abstract):

目的:复制2型糖尿病动物模型,并观察其肾脏病变及SnoN蛋白表达的变化。方法:SD大鼠随机分为正常对照组(NC)、高脂高糖组(HG)。高脂、高糖饮食12周后又随机分为高脂高糖对照组(HC)、糖尿病组(DM)。采用高脂、高糖饮食12周加小剂量链脲佐菌素注射(30 mg/kg)的方法复制2型糖尿病动物模型,成模大鼠随机分别于糖尿病8周(DM8)、12周(DM12)和16周(DM16)处死;Western印迹法检测肾皮质SnoN蛋白的水平;生物化学方法测血糖、血清胰岛素水平、甘油三酯、总胆固醇、血肌酐及24 h尿蛋白;光镜检查肾组织形态学改变。结果:DM组大鼠出现高血糖、高血脂、肾功能和肾组织形态学改变;HG组血清胰岛素水平、甘油三酯和总胆固醇明显升高,血糖与NC组相比差异无统计学意义;Western印迹检测发现DM8组肾组织中SnoN蛋白的表达明显低于NC组,且随病程进展逐渐减少,至DM16时减至NC组的64.20%。结论:成功复制了2型糖尿病动物模型;SnoN蛋白的表达变化可能参与了2型糖尿病肾脏病变的发生、发展过程。
Objective:To establish type 2 diabetic rat model and observe its renal lesions and SnoN protein expression.Methods: Sprague-Dawley rats were divided into normal control,high-fat and high-sucrose groups.High-fat and high-sucrose groups were then divided into high-fat and high-sucrose controls and diabetes mellitus groups after taking high-fat and high-sucrose diet for 12 weeks.The diabetic rats were injected with low dose of streptozotocin(30 mg/kg) and were killed at the 20th,24th,and 28th weeks respectively.The SnoN proteins in renal cortex were detected with Western blotting.Blood glucose,serum insulin concentration,trigly-ceride,total cholesterol,serum creatinine and 24 h urine protein were examined with biochemistry methods.The renal morphology on HE stained sections was checked with light microscope.Results: Diabetic rats showed hyperglycaemia,hyperlipemia,kidney morphological lesion and renal function changes.High-sucrose control group showed hyperinsulinemia and hyperlipemia,but the blood glucose was normal.Western blotting results showed that the expression of SnoN in diabetic renal tissues significantly decreased(P<0.01).Conclusions: Rat models of type 2 diabetes mellitus have been established successfully which can be used as a diabetic nephropathy model.The change of SnoN protein expression may play a role in the mechanisms of type 2 diabetic nephropathy.

关键词(KeyWords): 糖尿病,非胰岛素依赖型;糖尿病肾病;大鼠,Sprague-Dawley;转录共抑制因子SnoN
diabetes mellitus,non-insulin-dependent;diabetic nephrosis;rats,Sprague-Dawley;transcription co-repressor SnoN

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技攻关项目(编号:黔科字S[2007]1033号);; 贵州省高层次人才科研条件特助经费资助项目(编号:No.TZJF-2007年-44号);; 贵州省科技基金资助项目(编号:黔科合J字[2008]2167号)

作者(Author): 崔龙;刘瑞霞;李晓颖;石明隽;肖瑛;郭兵;张国忠;
CUI Long,LIU Ruixia,LI Xiaoying,SHI Mingjun,XIAO Ying,Guo Bing,ZHANG Guozhong(Department of Pathophysiology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

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DOI: 10.19367/j.cnki.1000-2707.2009.01.006

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