贵州医科大学学报

2011, v.36;No.152(05) 481-484

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胃泌素、5-羟色胺和生长抑素在急性酒精中毒大鼠胃窦的表达
Expression of Gas,5-HT and SS in Gastric Antrum of Rats with Acute Alcohol Intoxication

夏白娟;胡赟;李一欣;谢莉;
XIA Baijuan,HU Yun,LI Yixin,XIE Li (Department of Histology and Embryology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

摘要(Abstract):

目的:探讨急性酒精中毒对大鼠胃窦部胃泌素(Gas)、5-羟色胺(5-HT)和生长抑素(SS)免疫反应阳性细胞的影响。方法:正常雄性SD大鼠,随机分为正常组和实验组,实验组大鼠用白酒灌胃,在灌胃后不同时间段取胃窦组织,用免疫组织化学SABC法及图像分析法分析Gas、5-HT和SS阳性细胞的表达。结果:(1)与正常组比较,酒精中毒大鼠胃窦部Gas、5-HT阳性细胞数增多(P<0.05),以1 h组最为明显;SS阳性细胞数减少(P<0.05),以2 h组最为显著;(2)急性酒精中毒大鼠胃窦部Gas、5-HT阳性细胞灰度值均低于正常组(P<0.05),以1 h组最深;SS阳性细胞灰度值高于正常组(P<0.05),以2 h最明显。结论:在急性酒精中毒期间,Gas、5-HT在胃窦部表达增多,而SS分泌减少,提示这几种胃肠激素参与了酒精对胃黏膜的损伤过程。
Objective: To explore the effect of acute alcohol intoxication on Gas,5-HT and SS immunoreactively positive cells in gastric antrum of rats.Methods: Adult male SD rats were randomly divided into two groups: normal control group(group C) and experiment group(group E).Rats in group E were lavaged with alcohol.Then,the gastric antrum was obtained at different time points.The expression changes of Gas,5-HT and SS were studied with immunohistochemical SABC method and i-mage analysis technique.Results:(1)Compared with those of group C,Gas and 5-HT positive cell counts increased in gastric antrum of alcohol intoxication rats,especially in 1h group.However,SS positive cells decreased,especially in 2 h group(P<0.05).(2)Image analysis showed that mean grey degrees of Gas and 5-HT in group E were lower than those of group C(P<0.05),and those of 1 h group were the deepest.The mean grey degree of SS was stronger than that of group C,especially in 2 h group.Conclusions: The expression of Gas,5-HT increases and the secretion of SS decreases in rat gastric antrum during alcohol intoxication suggesting that these gastrointestinal hormones participate in adjusting the gastric mucosa damaging process caused by alcohol.

关键词(KeyWords): 酒精中毒,急性;胃泌素类;5-羟色胺;生长抑素;胃;免疫组织化学
alcoholic intoxication;gastrins;5-hydroxytryptamine;somatostation;stomach;immunohistochemistry

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基金项目(Foundation): 贵阳医学院青年基金K2007-41

作者(Author): 夏白娟;胡赟;李一欣;谢莉;
XIA Baijuan,HU Yun,LI Yixin,XIE Li (Department of Histology and Embryology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

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DOI: 10.19367/j.cnki.1000-2707.2011.05.012

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