贵州医科大学学报

2011, v.36;No.149(02) 111-114

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杜氏利什曼原虫无鞭毛体蛋白基因原核表达重组质粒的构建
Construction of Prokaryotic Expression Recombinant Plasmid of Amastin Gene of Leishmania donovani

李金福;陈建平;田玉;马莹;胡孝素;
LI Jinfu1,CHEN Jianping2,TIAN Yu2,MA Ying2,HU Xiaosu2(1.Department of Parasitology,Guiyang Medical College,Guiyang 550004,Guizhou,China;2.Department of Parasitology,School of Preclinical and Forensic Medicine,Sichuan University,Chengdu 610041,Sichuan,China)

摘要(Abstract):

目的:构建杜氏利什曼原虫无鞭毛体蛋白(amastin)编码基因的原核表达重组质粒pET-amastin。方法:提取杜氏利什曼原虫基因组DNA进行PCR扩增,将扩增出的无鞭毛体蛋白基因片段导入质粒载体pET-32a(+),构建原核表达重组质粒pET-amastin。结果:扩增出大小约550 bp的无鞭毛体蛋白基因,重组质粒pET-amastin经鉴定正确。结论:成功构建杜氏利什曼原虫无鞭毛体蛋白基因原核表达重组质粒pET-amastin。
Objective: To construct prokaryotic expression recombinant plasmid of amastin gene of Leishmania donovani.Methods: Amastin gene in nuclear DNA of L.donovani was isolated and amplified which was then cloned into prokaryotic expression vector pET-32a(+) to construct recombinant plasmid pET-amastin.Results: The amastin gene of 550bp was acquired by PCR.The recombinant plasmid pET-amastin was identified to be correct.Conclusion: The prokaryotic expression recombinant plasmid of amastin gene of L.donovani has been constructed successfully.

关键词(KeyWords): 利什曼原虫,杜氏;无鞭毛体蛋白;原核表达;基因克隆
Leishmania donovani;amastin;prokaryotic expression;gene cloning

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30771883);; 贵州省科学技术基金[黔科合J字(2008)2279号]

作者(Author): 李金福;陈建平;田玉;马莹;胡孝素;
LI Jinfu1,CHEN Jianping2,TIAN Yu2,MA Ying2,HU Xiaosu2(1.Department of Parasitology,Guiyang Medical College,Guiyang 550004,Guizhou,China;2.Department of Parasitology,School of Preclinical and Forensic Medicine,Sichuan University,Chengdu 610041,Sichuan,China)

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DOI: 10.19367/j.cnki.1000-2707.2011.02.002

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