大鼠脑内移植肾上腺髓质后单胺类神经递质及超微结构研究Study on Monoamine Neurtransmitters and Ultrastructure in the Striatum Transplantation of Adrenal Medulla in Rats
吴若秋;隋健英;左萍萍;高慧英;王振纲;
Wu Ruoqiu, et al Department of Neurosurgery
摘要(Abstract)：
对帕金森氏病模型鼠进行脑内移植肾上腺髓质,三月后用高效液相色谱测定停止旋转鼠的纹状体内的DA、HVA、Dopac、NE、5-HT、5-HIAA含量及对移植物进行光镜、电镜观察。结果,毁损侧纹状体内的DA含量降低(P<0.05),HVA降低35％,Dopac降低15％,移植后DA含量虽有增加,但无统计学意义,NE增加(P<0.05)5-HT、5-HIAA无变化,电镜下嗜铬细胞有神经元趋化及形成突触现象。
Homotranspantation of adrenal medulla into the striatum were carried out in 59 parkinson disease model rats. The changes in concentration of dopamine (DA), high vanillic acid (HVA), Dopac, norepinephrine (NE), 5-hydroxytryptamine (5-HT) and 5-hydroxyindole acetic acid (5-HIAA) in the striatum were studied High Effective Liquid Chromatography (HELC) before and after the tranplantion besides, the grafts were examined with light microscope and electroscanning microscope. The results revealed that DA concentration was decreased (P<0.05), HVA and Dopac were respectively decreased 35% and 15% in the lesioned striatum. After transplantation of adrenal medulla into the striatum, although the DA concentration was increased, yet it has no statistical significance. In addition, there were a marked elevation of NE concentration (P<0.05), while the concentration of 5-HT and 5-HIAA were unchanged in the striatum after the transplantation. The phenomenon of pheochromocyte neutropism and synaptic formation could be found under the eletroscanning microscope. This means that chromatocytes could developed along the neural line in the influence of external environment after the transplantation.
关键词(KeyWords)：
鼠;肾上腺髓质;自体移植;单胺类神经递质
rat; adrenal medulla; transplantation, autologous; monoamines neurohumor
基金项目(Foundation): 国家自然科学基金
作者(Author):
吴若秋;隋健英;左萍萍;高慧英;王振纲;
Wu Ruoqiu, et al Department of Neurosurgery
Email:
DOI: 10.19367/j.cnki.1000-2707.1991.04.002
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