贵州医科大学学报

2013, (01) 16-18+23

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银杏提取物对家兔缺血-再灌注心功能和心肌P-S及NOS表达的影响
The Effects of GBE on Cardiac Function and Expression of P-S and NOS during Ischemia/Reperfusion of Myocardium in Rabbits and Its Possible Mechanisms

张蓓;秦际德;刘兴德;
ZHANG Bei1,QIN Jide2,LIU Xingde2(1.Ultrasonic Examination Center,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004,Guizhou,China;2.Department of Cardiovascular Medicine,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004,Guizhou,China)

摘要(Abstract):

目的:观察银杏提取物(GBE)对心肌缺血-再灌注(I/R)家兔心功能及心肌血管内皮细胞中P-选择素(P-S)、一氧化氮合酶(NOS)表达的影响。方法:家兔(24只)随机分为3组,假手术组(Sham组)、心肌缺血再灌注组(I/R组)及GBE干预组(GBE组),记录各组家兔I/R 180 min时的心脏左室收缩压(LVSP)、左室舒张末压(LVEDP)和左室内压最大收缩-舒张变化速率(±dp/dtmax),用RT-PCR技术检测P-S、NOS。结果:与Sham组比较,I/R组LVSP和±dp/dtmax显著降低,LVEDP显著增高,有统计学意义(P<0.05);与I/R组比较,GBE组LVSP和±dp/dtmax显著增高,LVEDP显著降低,有统计学意义(P<0.05)。与I/R组比较,GBE心肌血管内皮细胞P-S表达显著减少,而NOS表达显著增多,有统计学意义(P<0.05)。结论:GBE能够改善心肌I/R家兔的心功能,这可能与下调血管内皮细胞P-S的表达、上调NOS的表达有关。
Objective: To investigate the effects of Ginkgo biloba extract(GBE) on cardiac function and expression of P-selection(P-S) and NOS in vascular endothelium cells of myocardium during ischemia/reperfusion(I/R) in rabbits.Methods: Twenty-four rabbits were randomly divided into 3 groups(8 in each): Sham-operated group(Sham),ischemia/reperfusion group(I/R),and GBE intervention group(GBE).Left ventricular systolic pressure(LVSP),left ventricular end-diastolic volume(LVEDP),and maximal changing rate of left ventricular inner(±dp/dtmax) of rabbits in 180min after I/R were recorded.Immunohistochemical method was used to detect expression of P-S ion and NOS in myocardium.Results: Of group I/R,LVSP and ± dp /dtmax were significantly lower,and LVEDP was significantly higher than those of group Sham(P<0.05);In group GBE,LVSP and ± dp /dtmax were remarkably higher,but LVEDP was remarkably lower than those in group I/R(P<0.05);Compared with that of group I/R,LVSP and ± dp /dtmax of group GBE increased remarkably,while LVEDP decreased remarkably(P<0.05).Compared with that of group I/R,P-S expression of group GBE decreased remarkably,while eNOS increased remarkably(P<0.05).Conclusion: GBE could improve cardiac function during myocardium I/R in rabbits.The mechanisms might be down regulation of P-S expression in vascular endothelium cells,and up regulation of eNOS expression.

关键词(KeyWords): 银杏提取物;心功能;再灌注损伤;P-选择素;一氧化氮合酶
Ginkgo biloba extract;cardiac function;reperfusion injury;P-selection;nitric-oxide synthase

Abstract:

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基金项目(Foundation): 贵州省高层次人才科研条件特助经费资助项目,TZJF(2006)12号

作者(Author): 张蓓;秦际德;刘兴德;
ZHANG Bei1,QIN Jide2,LIU Xingde2(1.Ultrasonic Examination Center,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004,Guizhou,China;2.Department of Cardiovascular Medicine,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004,Guizhou,China)

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