贵州医科大学学报

2019, v.44;No.222(03) 279-282+288

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卡铂对肝癌HepG2细胞影响的基因芯片分析
Microarray Analysis of Carboplatin on HepG2 Cells in Hepatocellular Carcinoma

岳萍;陈琳;黄文竹;胡祖权;王赟;邱炜;
YUE Ping;CHEN Lin;HUANG Wenzhu;HU Zuquan;WANG Yun;QIU Wei;School of Biology and Engineering,Guizhou Medical University;

摘要(Abstract):

目的:分析卡铂对肝癌HepG2细胞基因转录水平的影响。方法:用50 mg/L卡铂处理HepG2细胞48 h后,Trizol法抽提总RNA,利用基因芯片检测HepG2细胞基因在转录水平上的表达变化,分析获得差异基因;采用基因本体论(GO)分析差异转录基因分子功能、细胞组成及参与的生物过程,采用京都基因与基因组百科全书(KEGG)分析差异转录基因涉及的相关信号通路。结果:差异转录的基因共有1 474个(与对照组相比,变化倍数> 3),其中上调基因有520个,下调基因有954个;这些差异转录基因参与了酶结合、腺嘌呤核苷酸结合等相关功能,并与内质网、高尔基体、细胞骨架、锚定连接及细胞外间隙等有关;差异转录基因涉及淀粉和蔗糖代谢、抗坏血酸和醛酸代谢、MAPK通路、类固醇激素生物合成、补体与凝血级联途径等信号通路。结论:卡铂影响HepG2细胞中多个基因的转录,这些基因调控细胞多种功能。
Objective: The effect of carboplatin on gene transcription level of HepG2 cells was studied in this experiment to provide important information for the clinical treatment of carboplatin-based therapy against liver cancer. Methods: At 48 h after HepG2 cells were treated with 50 mg/L carboplatin,and total RNA was extracted by Trizol method. Gene chip was used to detect the expression changes of HepG2 genes at the transcription level,and differential genes were obtained. Genomic ontology( GO) was used to analyze the molecular function,cell composition and biological processes involved in differentially transcribed genes,and Kyoto gene and genome encyclopedia( KEGG) was used to analyze the relevant signal pathways involved in differentially transcribed genes. Results: There were1 474 differentially transcribed genes( compared with the control group,Fold Change > 3),of which520 were up-regulated and 954 were down-regulated. These differential genes involve all aspects of cell life,including a variety of physiological processes and signaling pathways. Conclusion: Carboplatin affects the transcription of multiple genes in HepG2 cells,which regulate multiple functions of the cells.

关键词(KeyWords): 肝肿瘤;卡铂;HepG2细胞;基因芯片;差异基因;GO分析;KEGG分析
liver neoplasms;carboplatin;HepG2 cells;microarray;differential genes;GO analysis;KEGG analysis

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基金项目(Foundation): 贵州省科技厅项目[黔科合LH字(2014)7083号];贵州省科技厅项目[黔科合LH字(2015)7326号]

作者(Author): 岳萍;陈琳;黄文竹;胡祖权;王赟;邱炜;
YUE Ping;CHEN Lin;HUANG Wenzhu;HU Zuquan;WANG Yun;QIU Wei;School of Biology and Engineering,Guizhou Medical University;

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