贵州医科大学学报

2020, v.45;No.243(12) 1417-1421

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三氧化二砷对体外人小肠癌HIC细胞增殖和凋亡的影响
Effects of Arsenic Trioxide on the Proliferation and Apoptosis of Human Small Bowel Cancer Cell Line HIC in Vitro

杨云洪;尹朝晖;张汝一;颜登国;徐伟;赵洋;李国胜;
YANG Yunhong;YIN Zhaohui;ZHANG Ruyi;YAN Dengguo;XU Wei;ZHAO Yang;LI Guosheng;Department of Colorectal-Anorectal Surgery,the Affiliated Hospital of Guizhou Medical University;

摘要(Abstract):

目的:探讨三氧化二砷(ATO)对体外人小肠癌HIC细胞增殖及凋亡的影响。方法:复苏冻存的人小肠癌HIC细胞培养至对数生长期,分别加10、15、20、40及60μmol/L ATO作为实验组,等量磷酸盐缓冲液(PBS)作为空白对照组,采用实时无标记细胞分析(RTCA)系统连续120 h动态监测各组人小肠癌HIC细胞的细胞指数(CI)值,并计算24和36 h时的细胞增殖抑制率;取对数生长期的人小肠癌HIC细胞,以分别加入10、15、20及40μmol/L ATO作为实验组,加等量PBS作为空白对照组,采用流式细胞仪检测各组人小肠癌HIC细胞48 h的凋亡情况。结果:ATO抑制人小肠癌HIC细胞增殖的抑制率随浓度及作用时间的延长而增高(P <0. 05),ATO诱导人小肠癌HIC细胞的凋亡率随浓度的增加而增高(P <0. 05)。结论:ATO可以在体外抑制人小肠癌HIC细胞的增殖,并诱导细胞凋亡的发生。
Objective: To investigate the effect of arsenic trioxide( ATO) on the proliferation and apoptosis of human small bowel cancer cell line HIC( human small intestine cancer) in vitro.Methods: Cryopreserved HIC cells were resuscitated and cultured till the logarithmic growth phase.HIC cells in the experimental group was treated with 10,15,20,40,and 60 μmol/L ATO,respectively. HIC cells in control group was given with the same amount of phosphate buffered saline( PBS). The real-time label-free cell analysis( RTCA) system was used to dynamically monitor the cell index( CI) values of HIC cells for 120 h. The inhibitory rates of cell proliferation were calculated at 24 and 36 h. Additionally,HIC cells at the logarithmic growth phase were stimulated with 10,15,20,and 40 μmol/L ATO as the experimental group for 48 h,and the same amount of PBS was added as the control group. Flow cytometry was used to detect HIC cellular apoptosis. Results: ATO-induced inhibitory rates in HIC cell proliferation remarkably increased in dose-and time-dependent manner( P < 0. 05),and ATO-induced apoptosis rates increased in dose-dependent manner( P < 0. 05).Conclusion: ATO can inhibit HIC cellular proliferation and induce cell apoptosis in vitro.

关键词(KeyWords): 细胞增殖;细胞凋亡;小肠癌;三氧化二砷;HIC细胞;抑制增殖
cell proliferation;apoptosis;small bowel cancer;arsenic trioxide(ATO);HIC cells;proliferation inhibition

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技计划项目[黔科合LH字(2016)7231]

作者(Author): 杨云洪;尹朝晖;张汝一;颜登国;徐伟;赵洋;李国胜;
YANG Yunhong;YIN Zhaohui;ZHANG Ruyi;YAN Dengguo;XU Wei;ZHAO Yang;LI Guosheng;Department of Colorectal-Anorectal Surgery,the Affiliated Hospital of Guizhou Medical University;

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DOI: 10.19367/j.cnki.2096-8388.2020.12.010

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