溶组织内阿米巴免疫粘附及免疫粘附血凝试验研究IAR and IAHA of E. histolytica
赵武麒;顾以铭;
Zhao Wuqi Cu Yiming Department of Parasitology
摘要(Abstract)：
应用正交设计试验方法探讨了影响阿米巴免疫粘附玫瑰花形成的诸多因素,发现抗体浓度,补体浓度、孵育温度及指示人红细胞浓度均对成花率有显著影响,其中孵育温度为主要因素。还用本法确定了免疫粘附玫瑰花试验的佳化方案。另外,用绵羊红细胞作载体结合阿米巴可溶性抗原,成功地进行了微量免疫粘附血凝试验。该方法灵敏、可靠、特界性强,所用抗原试剂量少,有实用和推广价值。
The several factors which influence on IAR test are analysed by orthogonal experimental design in this paper. The results of analysis suggested that some factors have obvious influence on the percentage of IAR such as the concentration of the Ab., complement, HuRBC and incubating temperatures etc., The incubating temperature is main factor. The optimal scheme of IAR is determined by this method. The IAHA of amebae is performed successfully by using sRBC or rRBC sensitized with soluble Ag. of E.histolytica. It is more sensitive, reliable and specific.
关键词(KeyWords)：
溶组织内阿米巴;免疫粘连反应;玫瑰花结形成
cntamoeba histolytica; immune adherence reaction; rosette formation
基金项目(Foundation):
作者(Author):
赵武麒;顾以铭;
Zhao Wuqi Cu Yiming Department of Parasitology
Email:
DOI: 10.19367/j.cnki.1000-2707.1991.04.012
参考文献(References)：
- 1． 凌天翼,等.免疫粘附玫瑰花试验条件初探.贵阳医学院学报 1988;13(2) :174～180．
- 2． 陶义训,等主编.临床免疫学检验(下册).第一版.上海:上海科学技术出版社,1986:21～26,47～59,75～84．
- 3． 杨贵贞,主编.医用免疫学.第一版,长春:吉林人民出版社,1980:345～355． 379～380．
- 4． 王玉龙.等,正常人红细胞花环水平及其影响因素的初步研究.免疫学杂志 1985;1(1) :73．
- 5． Davis, L J, et al. The adhesior phenomenon, a specific serological reaction occurring in trypanosomiasis. Trans. R. Soc. Trop. Med. Hyg. 1927; 21: 113．
- 6． Nelson, R.A.The immune-adherence phenomenon. An immunologically specific reaction betweer microorganisms and erythrocytes leading to enhanced phagocytosis. Science 1953; 118: 733．
- 7． Nishioka, K. Measurements of complement by agglutination of human erythrocytes reacting in immune adherences. J Immunol 1963; 90: 86．
- 8． Nishioka K, et al. Components of guinea pig complement. I. separation of a serum fraction essential for immune hemolysis and immune adherence. J Exp Med 1963; 118: 767．
- 9． 范维珂.检测细胞表面抗原体的玫瑰花形成试验(综述).上海免疫学杂志1986;6:250～252．
- 10． Ito M, et al. Immune adherence hemagglutination test as a new sensitive method for titration of animal virus antigens and antibodies. Jap J Med S Biol 1966; 19: 109．
- 11． Siegel I, et al. The red-cell immune system. The Lancet 1981; 12: 556．
- 12． Brown H C, et al. Studies in trypanosomiasis. Ⅱ. Observations on the red cell adhesion test. Trans R Soc Trop Med Hyg 1938; 32: 209．
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