苦瓜提取物对小白鼠致畸试验Teratogenesis Test of Momordica Charantia Extract in Mice
李远慧;覃国芳;高晓勤;
Li yuanhui, et al Department of Histology and Embryology
摘要(Abstract)：
本文对妊娠6～14天的雌鼠灌胃苦瓜提取物,以确定该药有无胚胎毒和致畸作用。结果显示低剂量范围(2.5ml/kg)苦瓜提取物对小白鼠无致畸和胚胎毒作用。高剂量组(15ml/kg)活胎数、胎重和胎鼠外形、内脏异常数与阴性对照组无差异,而孕鼠体重、死胎数和骨骼异常数与阴性对照组有差异。提示可能高剂量苦瓜提取物有轻度胚胎毒作用,但无致畸作用。五氯酚钠阴性对照组出现明显胚胎毒和致畸作用。
Momordica charantia extract was used in 23 pregnant mice by gavage to determine whether it has embryotoxic and teratogenic effects. The mice were divided into two groups (13 mice in dose of 2.5ml/kg/d10, mice in dose of 15ml/kg/d). In conclusion: In lower dosage group, M. Charantia Lnn extract showed no embryotoxic and teratogenic effects. The live mbryo number, embryo weight and the anomalies of embryo shape and viscera in high dosage group revealed no statistically significant differences, as comparing with negative control group. The pregnant mice weight, dead embryo number and skeleton anomalies revealed a significant difference with negative control group. It suggests that the M. charantia extract in high dose showed a lesser embryotoxic effect, but had not teratogenic effect in mice. The pentachlorophenol sodium salt revealed a marked embryotoxic and teratogenic effects in the positive control group.
关键词(KeyWords)：
苦瓜;抗精子发生剂;致畸胎物
momordica charantia; antispermatogenic agents; teratogens
基金项目(Foundation):
作者(Author):
李远慧;覃国芳;高晓勤;
Li yuanhui, et al Department of Histology and Embryology
Email:
DOI: 10.19367/j.cnki.1000-2707.1991.04.015
参考文献(References)：
- 1． 覃国芳.(84001对睾丸、附睾超微结构的影响--扫描电镜及透射电镜观察(84001抗精作用研究之三).贵阳医学院学报 1986;11(3) :191～194．
- 2． 徐幼云,等.环境卫生工作手册.第 二版.北京:人民卫生出版社,1983:296～299．
- 3． Wilson J G. Teratology Principles and Techniques 1975: 262～277．
- 4． 杜卓民,主编.实用组织学技术.第一版.北京:人民卫生出版社,1982:254～257．
- 5． 覃国芳,等.新除草剂“草甘磷”的毒性研究.环境科学 1984;2:52～54．
- 6． 许庭良,等.古尼拟青霉对小白鼠的致畸试验.贵阳医学院学报 1988;13(4) :440～443．
- 7． 杨克敌.化学物质致畸作用的研究进展(综述).国外医学(卫生学分册)1981;8(6) :338～341．
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