卢步峰;阮清云;杨蜀莲;俞良力;贾淑兰;杨默;杨青;黄詒森;
Lu Bufeng, et al Department of Biochemistry, Zhenjiang Medical College

• 1: 镇江医学院生化教研室
• 2:北京协和医院神经科
• 3:北京协和医院神经科
• 4:北京协和医院神经科

摘要(Abstract)：

本实验采用闭塞大鼠四条血管的脑缺血动物模型,在全脑缺血15min和缺血15min再灌注3h后分别观察海马、下丘脑和额叶大脑皮层内P物质和单胺类递质的动态变化。结果示:无论在脑缺血或脑再灌注时上述物质的含量均发生改变,但同一物质在不同脑区内以及相同脑区内不同物质的变化其程度不等,方向也不一致。提示不同脑区对脑缺血和脑再灌注可作出不同的反应。
The experiment was performed in a four vossels occlusion model of rats. The changes of substance P and monoamine transmitters in hippocampus, hypothalamus and cortex were observed respectively during 15 min complete cerebral ischemia and 15 min complete cerebral ischemia followed by reperfusion for 3h. The results indicated that the changes of substance P and monoamine transmitters varied in different areas of the rat brain and these two kinds of substance themselves showed different changes in a same area. The results showsed that the response in different cerebral areas to cerrebral ischemia and cerebral reperfusion were different. Substance P have been found to inter- act and/or co-exist with classical transmitters, such as monoamines, in same neuronal bodies or terminals. From the data presented in this paper we propose that these two kinds of substance might have some relation in the pathoph-y seiology of cerebral ischemia and cerebral reperfusion.

关键词(KeyWords)： 大鼠;脑缺血;海马;下丘脑额叶;P物质;单胺类递质
rat; cerebral ischcmia; hippocampus; hypothalamus; frontal lobe: substance P; monoamine transmitters

Abstract:

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作者(Author): 卢步峰;阮清云;杨蜀莲;俞良力;贾淑兰;杨默;杨青;黄詒森;
Lu Bufeng, et al Department of Biochemistry, Zhenjiang Medical College

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参考文献(References)：

• 1． 陈娟,等.P物质放射免疫测定法.中国医学科学院学报 1986,8 (1) :7～12．
• 2． Shigeo M, et al. Rapid determination of norepinephrine, dopamine, serotomine, their precursor amino acids, and related metabolites in discrete brain areas of mice within ten minute by HPLC with electrochemical detection. J Neurochem 1988; 50 (2) : 473-479．
• 3． Pernew B. Substance P. Pharmacol Rev 1983; 35 (2) : 85-114．
• 4． Brownstein MJ. Regingal distribution of substance P in the brain of the rat. Btrain Res 1976; 116: 209．
• 5． Ljiungdahl A, et al. Distribution of Substance P-like immuno-reactivity in the central nervous system of rat. Ⅱ. Light microscopic localization in relation to catecholamine-containing neurons.N euroscience 1978: 3: 945-76．
• 6． Caranikas A, et al. Effect of synthetic substance P on central monoaminergie mechanisms. In Substance P (Nobel Symposium 37) ed. New York: U S von Euler and B Pernow, Raven Press, 1977: 201-205．
• 7． Garcia-Sevilla JA, et al. Opposite effects of naloxone on substance P-induced change in brain DOPA synthesis and in loco motor activity in rats. J Neural Transm 1979; 45: 185--93．
• 8． Hokfelt T, et al. Immunohistochemical evidence of substance-P-like immunoreactivity in some 5-hydroxytrypamine-containing neurons in the rat central nervous system. Neuroscience 1978; 3: 517～38．
• 9． 匡培根,等.脑缺血时脑内单胺类件、经介质的动态变化.中国神经精神病杂志 1983,9(5) :287。
• 10． Harrison MJG, et al. Effect of experimental ischemia on neurotransmitter amines in the gerbil brain. Stroke 1979; 10: 165．

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