贵州医科大学学报

2019, v.44;No.222(03) 289-292

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低温联合右美托咪啶对兔心肌细胞缝隙连接蛋白43分布的影响
Effects of Hypothermia Combined with Dexmedetomidine on the Distribution of Gap Junction Protein Connexin 43 in Cardiomyocytes of Rabbits

安丽;李惠;刘艳秋;吕洁;姚茵;易菁;王子君;高鸿;
AN Li;LI Hui;LIU Yanqiu;LV Jie;YAO Yin;YI Jing;WANG Zijun;GAO Hong;Department of Anesthesiology,the Affiliated Hospital of Guizhou Medical University;School of Anesthesiology,Ghuizhou Medical University;

摘要(Abstract):

目的:探讨低温条件下使用右美托咪啶对兔离体心脏心肌细胞缝隙连接蛋白43(Cx43)分布的影响。方法:18只健康成年新西兰家兔,建立Langendorff离体心脏灌注模型,造模后随机分为对照组(C组)、低温组(L组)和低温及右美托咪啶组(LD组),C、L组将离体心脏分别置于37℃及32℃K-H液持续灌注60 min,LD组将离体心脏置于含25μg/L右美托咪啶的K-H液中持续灌注60 min;取各组左心室的前壁心肌组织采用免疫组织化学检测Cx43蛋白分布情况。结果:与C组比较,L组及LD组Cx43蛋白阳性着色颗粒减少,并有端端链接转向侧侧链接的现象,排列紊乱; Cx43蛋白平均光密度值下调(P <0. 01); L组和LD组心肌细胞Cx43蛋白平均光密度值比较,差异无统计学意义(P> 0. 05)。结论:右美托咪啶不加重低温条件下心肌细胞Cx43蛋白的分布改变。
Objective: To investigate the effects of hypothermia combined with dexmedetomidine on the distribution of gap junction protein connexin 43( Cx43) in isolated heart cardiomyocytes of rabbit.Methods: 18 NewZealand healthy adult rabbits weighing up to 2. 0 ~ 2. 5 kg were randomly divided into three groups( n = 6) after Langendorff isolated heart perfusion model was established: control group( group C),hypothermia group( group L),and hypothermia + dexmedetomidine group( group LD).The control group( group C) underwent continuous perfusion with Krebs-Henseleit solution( 37 ℃)for 60 min. The hypothermia group( group L) underwent continuous perfusion with Krebs-Henseleit solution( 32 ℃) for 60 min,and the hypothermia plus dexmedetomidine( group LD) underwent continuous perfusion with Krebs-Henseleit solution and dexmedetomidine for 60 min. The distribution of Cx43 protein in the anterior wall of the left ventricle of all groups was detected by immunohistochemistry. Results: Group L and group LD were compared with group C,there was a tendency for the proteins localized at end-to-end contact sites of ventricular cardiomyocytes to localize at side-to-side contact sites,and the distribution was messy,the average optical density of Cx43 protein decreased( P <0. 01). Comparison of average optical density of Cx43 protein between the groupL and groupLD showed that the difference had no statistical significance( P > 0. 05). Conclusion: Under 32 ℃ temperature condition,cardiomyocytes of rabbit isreduced protein distribution of CX43,which may be one of the mechanisms of the hypothermia inducing arrhythmia in rabbits. However,the use of dexmedetomidine in hypothermic conditions will not aggravate these hypothermia-related changes so as that there is no increased risk of arrhythmia.

关键词(KeyWords): 低温;右美托咪啶;缝隙连接蛋白Cx43;心肌细胞;离体心脏;模型,动物
hypothermia;dexmedetomidine;connexin 43;cardiomyocytes;isolated heart;model,animals

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基金项目(Foundation): 贵州省科技厅联合基金[黔科合LH字(2016)7252号]

作者(Author): 安丽;李惠;刘艳秋;吕洁;姚茵;易菁;王子君;高鸿;
AN Li;LI Hui;LIU Yanqiu;LV Jie;YAO Yin;YI Jing;WANG Zijun;GAO Hong;Department of Anesthesiology,the Affiliated Hospital of Guizhou Medical University;School of Anesthesiology,Ghuizhou Medical University;

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