贵州医科大学学报

2004, (04) 333-334

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反相高效液相色谱法测定金莲花胶囊中牡荆苷的含量
Determination of Vitexin in Flos Trollii Capsules by RP-HPLC

王萍,刘智,王丽,李雯
WANG Ping 1, LIU Zhi 2, WANG Li 2, LI Wen 2 (1. Guizhou Center for Drug Evaluation and Certification, Guiyang 550004,China; 2. Guizhou Eakan Pharmaceutical Co., Ltd, Guiyang 550001,China )

摘要(Abstract):

目的 :建立金莲花胶囊中牡荆苷的含量测定方法。方法 :采用高效液相色谱法 ,使用HypersilC1 8ODS色谱柱 ,以乙腈 - 1 %乙酸溶液 (1 5∶85 )为流动相 ,流速为 1 0ml/min ,检测波长为 340nm。结果 :牡荆苷在8 0 7~ 80 7mg/L范围内呈良好的线性关系 (r =0 9999) ,平均回收率为 1 0 2 4 % ;RSD =1 7%。 结论 :用高效液相色谱法测定金莲花胶囊中牡荆苷的含量 ,具有灵敏、准确、专属性强、重现性好的特点
Objective: To establish a HPLC method for determination of vitexin in Flos Trollii capsules. Methods: The system consisted of ODS silica column and acetonitrile 1% acetic acid solution (15∶85) as the mobile phase. The elution with a flow rate of 1 0 ml·min -1 was detected at the wavelength of 340 nm. Results: Vitexin showed a good linear relationship in the range of 8 07~80 7 mg·ml -1 ( r =0 999 9) and the average recovery rate was 102 4%. RSD was 1 7%. Conclusion: The method is sensitive, specific, and accurate with a good repeatability and can be used for the quality control of Flos Trollii capsules.

关键词(KeyWords): 金莲花胶囊;牡荆;色谱法,高压液相
Trollius chinesis Bge; vitexin; chromatography, high pressure liquid

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作者(Author): 王萍,刘智,王丽,李雯
WANG Ping 1, LIU Zhi 2, WANG Li 2, LI Wen 2 (1. Guizhou Center for Drug Evaluation and Certification, Guiyang 550004,China; 2. Guizhou Eakan Pharmaceutical Co., Ltd, Guiyang 550001,China )

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DOI: 10.19367/j.cnki.1000-2707.2004.04.018

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