贵州医科大学学报

2019, v.44;No.230(11) 1310-1315

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4种长链非编码RNA在重度冠心病患者外周血单个核细胞中的表达及临床意义
Clinical Significance and Expression of Four IncRNAs in Peripheral Blood Mononuclear Cells of Patients with Severe Coronary Heart Disease

游赣花;龙向淑;宋方;黄晶;田茂波;肖燕;邓世燕;吴强;
YOU Ganhua;LONG Xiangshu;SONG Fang;HUANG Jing;TIAN Maobo;XIAO Yan;DENG Shiyan;WU Qiang;Medical College of Guizhou University;Guizhou Institute for Food and Drug Control;Department of Cardiology, Guizhou Provincial People's Hospital;Department of Cardiology, People's Hospital of Guizhou University;

摘要(Abstract):

目的:探讨长链非编码RNA(lncRNA)肺腺癌转移相关转录本1(MALAT1)、核糖核酸牛磺酸上调基因1(TUG1)、Ezrin反义RNA1(EZR-AS1)及lncRNA n342419 (MANTIS)在重度冠心病(CHD)患者外周血单个核细胞(PBMC)中的差异表达及临床意义。方法:选取确诊为重度CHD患者35例作为CHD组,同期无CHD的体检人群38例为对照组;收集所有研究对象的临床特征资料,并抽取空腹静脉血8 mL,分别采用全自动生化分析仪和实时定量PCR(RT-qPCR)检测总胆固醇(CHOL)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)及4种lncRNA的表达,采用受试者工作特征(ROC)曲线分析4种lncRNA对重度CHD患者的诊断价值。结果:CHD组患者HDL-C水平比对照组体检人群明显降低(P<0.01),MALAT1、TUG1及EZR-AS1的表达比对照组体检者明显升高,但MANTIS的表达则明显降低(P<0.01);ROC曲线结果显示,MALAT1、TUG1、EZR-AS1、MANTIS的AUC分别为0.851(95%CI为0.764~0.938,P<0.01)、0.768(95%CI为0.662~0.875,P<0.01)、0.884(95%CI为0.811~0.957,P<0.01)及0.807(95%CI为0.707~0.907,P<0.01)。结论:MALAT1、TUG1、EZR-AS1及MANTIS在重度CHD患者PBMC中差异性表达与CHD严重程度相关。
Objective: To investigate the expression and clinical significance of long noncoding RNA(lncRNA) metastasis-associated lung adenocarcinoma transcript 1(MALAT1), taurine up-regulated 1(TUG1), Ezrin antisense RNA1(EZR-AS1), and lncRNA n342419(MANTIS) in peripheral blood mononuclear cells(PBMC) of patients with severe coronary heart disease(CHD). Methods: The experiment was divided into two groups, the control group(38 cases) and CHD group(diagnosed 35 cases). Collecting clinical data of all subjects and drawing 8 ml venous blood on an empty stomach to analyze on automatic biochemical analyzer and qRT-PCR for following expressions of CHOL, HDL-C, LDL-C and four types of IncRNA. Adopting subjects ROC curve to analyze diagnostic value of four types of IncRNA on severe CHD patients. Results: Compared with the control group, the expression level of HDL-C of CHD was significantly lowered(P<0.01),MALAT1, TUG1, and EZR-AS1 increased significantly, while the expression of MANTIS decreased sharply(P<0.01). The results of ROC curve showed that the AUC of MALAT1, TUG1, EZR-AS1, and MANTIS were 0.0851(95%CI was 0.764~0.938, P<0.01), 0.768(95%CI was 0.662~0.875, P<0.01), 0.884(95%CI was 0.811~0.957, P<0.01), and 0.807(95%CI was 0.707~0.907, P<0.01), respectively. Conclusion: The differential expression of MALAT1, TUG1, EZR-AS1, and MANTIS in PBMC of patients with severe CHD were closely related with severity of CHD and have the potential to be biomarkers for diagnosis and evaluation of CHD.

关键词(KeyWords): 冠心病;外周血;单个核细胞;长链非编码RNA;肺腺癌转移相关转录本1;核糖核酸牛磺酸上调基因1;Ezrin反义RNA1;lncRNA n342419
coronary heart disease;peripheral blood;mononuclear cells;long noncoding RNA;metastasis-associated lung adenocarcinoma transcript 1;taurine up-regulated 1;ezrin antisense RNA1;lncRNA n342419

Abstract:

Keywords:

基金项目(Foundation): 贵州省高层次创新型人才培养计划[黔科合人才(2016)4023];; 贵州省科技平台及人才团队计划项目[黔科合平台人才(2017)5405]

作者(Author): 游赣花;龙向淑;宋方;黄晶;田茂波;肖燕;邓世燕;吴强;
YOU Ganhua;LONG Xiangshu;SONG Fang;HUANG Jing;TIAN Maobo;XIAO Yan;DENG Shiyan;WU Qiang;Medical College of Guizhou University;Guizhou Institute for Food and Drug Control;Department of Cardiology, Guizhou Provincial People's Hospital;Department of Cardiology, People's Hospital of Guizhou University;

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DOI: 10.19367/j.cnki.1000-2707.2019.11.013

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