贵州医科大学学报

2019, v.44;No.227(08) 869-874

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长链非编码RNA ITGβ2-AS1对胰腺癌细胞增殖和侵袭及迁移能力的影响
Effect of Long Non-coding RNA ITGβ2-AS1 on Proliferation, Invasion and Migration of Pancreatic Cancer Cells

陈世裕;喻超;潘耀振;陈玲;李琳;杨哲豪;吕彦霖;邓路;孙诚谊;
CHEN Shiyu;YU Chao;PAN Yaozhen;CHEN Ling;LI Lin;YANG Zhehao;LV Yanlin;DENG Lu;SUN Chengyi;Guizhou Medical University;Department of Hepatobiliary Surgery, the Affiliated Hospital of Guizhou Medical University;Key Laboratory of Liver, Gallbladder, Pancreas and Spleen of Guizhou Medical University;Guizhou Institute of Hepatobiliary,Pancreatic and Spleen Diseases;

摘要(Abstract):

目的:探讨长链非编码RNA(LncRNA)整合素β2 (ITGβ2)-AS1在体外对人胰腺癌细胞增殖、侵袭及迁移能力的影响及其机制。方法:采用癌症基因组图谱(TCGA)公共数据库分析ITGβ2-AS1在胰腺癌组织及癌旁组织中的表达,通过qPCR实验检测ITGβ2-AS1分别在人正常胰腺导管上皮细胞及人PC细胞株AsPC-1、CFPAC-1、PANC-1、MIA PaCa-2、Capan-2、BxPC-3、Hs766T及SW1990细胞中的表达,利用慢病毒载体稳定转染ITGβ2-AS1最高的表达细胞,采用CCK-8和平板克隆实验检测ITGβ2-AS1对PANC-1细胞增殖能力的影响;通过细胞划痕及Transwell实验检测ITGβ2-AS1对PANC-1细胞侵袭及迁移能力的影响,qPCR及Western blot实验检测干扰ITGβ2-AS1后PANC-1细胞中ITGβ2的基因表达情况。结果:ITGβ2-AS1在胰腺癌组织中的表达明显高于癌旁组织,且在各胰腺癌细胞株中的表达显著高于人正常胰腺导管上皮细胞,PANC-1中表达最高,差异有统计学意义(P<0.05);干扰ITGβ2-AS1后,PANC-1细胞的增殖、迁移及侵袭能力显著降低,ITGβ2的表达水平显著下降,差异有统计学意义(P<0.05)。结论:LncRNA ITGβ2-AS1在胰腺癌中高表达,干扰其表达可抑制胰腺癌细胞的增殖、侵袭及迁移能力和抑制ITGβ2的转录及翻译过程。
Objective: To investigate the effect of long non-coding RNA integrin β2(ITGβ2)-AS1 on proliferation,invasion and migration of human pancreatic cancer cells in vitro and to explore its mechanism preliminarily.Methods: The expression of ITGβ2-AS1 in pancreatic cancer tissues and adjacent tissues was analyzed and compared based on the TCGA database and the expression of ITGβ2-AS1 in human normal pancreatic ductal epithelial cells and pancreatic cancer cells was detected by qPCR assay; The pancreatic cancer cell line PANC-1 was stably transfected with lentiviral vector and the effect of ITGβ2-AS1 on proliferation of pancreatic cancer cells was detected by CCK-8 and colony formation assays; The effect of ITGβ2-AS1 on invasion and migration of pancreatic cancer cells was detected by wound healing and Transwell assays; The expression of ITGβ2 after interfering with ITGβ2-AS1 was detected by qPCR and Western blot assays.Results: The expression of ITGβ2-AS1 in pancreatic cancer tissues was significantly higher than that in adjacent tissues,and the expression of ITGβ2-AS1 in pancreatic cancer cell lines was significantly higher than that in human normal pancreatic ductal epithelial cells(P<0.05). After interfering with ITGβ2-AS1,the proliferation,migration and invasion ability of pancreatic cancer cells decreased significantly(P<0.05) and the expression level of ITGβ2 decreased significantly(P<0.05).Conclusion:Long non-coding RNA ITGβ2-AS1 is highly expressed in pancreatic cancer,interfering its expression may inhibit the proliferation,invasion and migration of pancreatic cancer cells and inhibit the transcription and translation process of ITGβ2.

关键词(KeyWords): 长链非编码RNA;整合素β2;胰腺癌;增殖;侵袭;迁移
long non-coding RNA;ITGβ2;pancreatic carcinoma;proliferation;invasion;migration

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81860505,81860506);; 贵州省科学技术厅—贵州医科大学附属医院联合基金[黔科合LH字(2016)7229];; 贵州省肝胆外科临床医学研究中心[黔科合平台人才(2017)5404];; 贵州省高层次创新人才培养计划“十”层次人才,黔科合平台人才[(2016)5647];; 贵州省科学技术厅—贵阳医学院院士工作站肝胆外科分站[黔科合院士站(2015)4013];; 贵州省孙诚谊“肝胆胰脾疾病诊治”导师工作室,黔教研合[GZS(2016)09];; 贵州省第四批人才基地—贵州省外科人才培养基地[黔省专合字(2012)94];; 贵州省科技支撑计划项目[黔科合SY字(2015)3047]

作者(Author): 陈世裕;喻超;潘耀振;陈玲;李琳;杨哲豪;吕彦霖;邓路;孙诚谊;
CHEN Shiyu;YU Chao;PAN Yaozhen;CHEN Ling;LI Lin;YANG Zhehao;LV Yanlin;DENG Lu;SUN Chengyi;Guizhou Medical University;Department of Hepatobiliary Surgery, the Affiliated Hospital of Guizhou Medical University;Key Laboratory of Liver, Gallbladder, Pancreas and Spleen of Guizhou Medical University;Guizhou Institute of Hepatobiliary,Pancreatic and Spleen Diseases;

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DOI: 10.19367/j.cnki.1000-2707.2019.08.001

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