贵州医科大学学报

2006, (01) 51-53

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高效液相色谱法测定复肾宁胶囊中栀子苷的含量
Determination of Geniposide in Fushenning Capsule by HPLC

涂波;王丽;
TU Bo~1,WANG Li~2(1.Department of Chemistry,Guiyang Medical College,Guiyang 550004,China;2.Guiyang Maidike Medical Science and Technology Development Institute,Guiyang 550002,China)

摘要(Abstract):

目的:探寻复肾宁胶囊中栀子苷的含量测定方法。方法:采用高效液相色谱法测定栀子苷的含量,色谱柱Hypersil ODS2柱,流动相为乙腈-水(12∶88),流速1.0 m l/m in,检测波长238 nm。结果:该方法线性关系良好,平均加样回收率为99.07%,RSD=1.00%(n=6)。结论:本方法简便、快速、精密度高、分离度良好,可用于复肾宁胶囊的质量控制。
Objective: To establish a method to determine the content of geniposide in Fushenning capsule. Methods: HPLC was adopted.The system consisted of Hypersil ODS2 column and acetonitrile-water(12∶88)as mobile phase with a flow rate of 1.0 ml/min and the UV detection wavelength was 238 nm.Results: A good linear correlation was found.The average recovery rate was 99.07%,RSD=1.00%(n=6). Conclusion: This method is accurate and could be used for quality control of the capsule.

关键词(KeyWords): 植物制剂;色谱法,高压液相;栀子苷;复肾宁胶囊
plant preparations;chromatography,high pressure liquid;geniposide;Fushenning capsule

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作者(Author): 涂波;王丽;
TU Bo~1,WANG Li~2(1.Department of Chemistry,Guiyang Medical College,Guiyang 550004,China;2.Guiyang Maidike Medical Science and Technology Development Institute,Guiyang 550002,China)

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DOI: 10.19367/j.cnki.1000-2707.2006.01.016

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