贵州医科大学学报

1980, (02) 11-12

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鸡疟原虫(PIasmodium gallinaceum)动合子的离体培养
Cultivation in vitro of ookinetes of Plasmodium gallinaceum

毛克强;王宝林;
Mao Ke--chiang;Wang Bao--lin Department of Parasitology

摘要(Abstract):

<正> 疟疾免疫学研究和企图利用子孢子虫苗预防疟疾需要获得大量的子孢子。这显然不能依靠解剖按蚊涎腺中的子孢子,必须将人体红细胞内的配子体离体培养成为子孢子。这是国内外急待解决的问题。国外曾报告采用各种培养基将鼠疟原虫配子体培养成为动合子。我们于1976年用Eagle’sMEM营养液和原代鸡胚肌皮单层细胞加入Eagle’sMEM营养液培养鸡疟原虫配子体成为动合子。
we succeeded in 1976 in the cultivation of Plasmodium gallinaceum from game- tocyte to ookinete. The basic medium used was Eagle's MEM prepared according to the method of Lennet et al (1969), with 10% foetal calf serum added and adjust ed to pH 7. 4. Two methods were used: (1) 0. 5ml chicken blood with contained gametocytes was added to 2ml Eagle's MEM; (2) 2ml Eagle's MEM was added to the primary culture of chicken emdryo monolayer cells and then 0. 5ml chickekn blood with contained gametoeytes was inoculated. These were maintained at 27℃. In the three cultivations ookinetes (2--26/slide) were demonstrated after 20--45 hrs incubation while the another four cultivations were negative.

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作者(Author): 毛克强;王宝林;
Mao Ke--chiang;Wang Bao--lin Department of Parasitology

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DOI: 10.19367/j.cnki.1000-2707.1980.02.005

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