视网膜脱离伴脉络膜脱离者外周血炎症相关细胞因子的含量The Peripheral Blood Levels of Helper T Lymphocyte 1 Cytokines in Patients with Rhegmatogenous Retinal Detachment Associated with Choroidal Detachment
于旭辉;葛红岩;张蕾;宋晗;遇颖;王宇虹;
YU Xuhui1,GE Hongyan1,ZHANG Lei1,SONG Han1,YU Ying1,WANG Yuhong2(1.Department of Ophthalmology,The First Clinical College of Harbin Medical University,Haerbin 150001,Heilongjiang,China;2.Department of Neurology,The First Clinical College of Harbin Medical University,Haerbin 150001,Heilongjiang,China)
摘要(Abstract):
目的:系统监测视网膜脱离伴脉络膜脱离(脉脱型网脱)患者外周血中促炎细胞因子IL-12和IL-18,抑炎细胞因子IL-4和IL-10以及炎细胞效应因子IFNγ-和TNFα-的含量。方法:选取脉脱型网脱患者23例,正常对照组19例,分别采集患者和健康对照者的外周静脉血,用酶联免疫吸附方法(EILSA)检测IL-12、IL-18、IL-4、IL-10、TNFα-和IFNγ-的表达水平。结果:脉脱型网脱患者外周血中IL-12及IFNγ-、TNFα-的含量显著高于对照组(P<0.05),IL-10显著低于对照组(P<0.05),IL-18和IL-4的变化不明显(P>0.05)。结论:在脉脱型网脱中,促炎因子IL-12表达增高,抑炎因子IL-10的表达降低,同时效应炎性因子TNFα和IFNγ均显著增高,表明Th1介导的炎性损伤在脉脱型网脱的病程中有重要作用。
Objective: To systemically monitor levels of cytokines including proinflammatory cytokines IL-12 and IL-18,antinflammatory cytokines IL-4 and IL-10,and inflammatory cell effect cytokines IFN-γ and TNF-α in peripheral blood of patients with rhegmatogenous retinal detachment associated with choroidal detachment.Methods: Serum cytokines(intergeron IFN-γ,TNF-α,interleukin IL-4,IL-10,IL-18 and IL-12) levels in 23 patients suffering from rhegmatogenous retinal detachment associated with choroidal detachment and 19 healthy controls were measured by using sandwich ELISA.Results: Higer levels of IFN-γ,TNF-α and IL-12 and lower levels of IL-10 were found in serum samples from patients than in those of controls(P<0.05).No difference was found between IL18 or IL-4 levels of patients and healthy controls.Conclusion: Inflammatory damage mediated by helper T lymphocyte 1 might play an important role in the developing course of retinal detachment associated with choroidal detachment.
关键词(KeyWords):
视网膜脱离;细胞因子类;酶联免疫吸附测定;视网膜脱离伴脉络膜脱离
retinal detachment;cytokines;enzyme-linked immunosorbent assay;choroidal detachment associated with retinal detachment
基金项目(Foundation): 黑龙江省卫生厅医学科研专题资助项目项目编号2006-018;; 黑龙江省攻关国际合作项目项目编号WC05C04
作者(Author):
于旭辉;葛红岩;张蕾;宋晗;遇颖;王宇虹;
YU Xuhui1,GE Hongyan1,ZHANG Lei1,SONG Han1,YU Ying1,WANG Yuhong2(1.Department of Ophthalmology,The First Clinical College of Harbin Medical University,Haerbin 150001,Heilongjiang,China;2.Department of Neurology,The First Clinical College of Harbin Medical University,Haerbin 150001,Heilongjiang,China)
Email:
DOI: 10.19367/j.cnki.1000-2707.2007.05.015
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文章评论(Comment):
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- 视网膜脱离
- 细胞因子类
- 酶联免疫吸附测定
- 视网膜脱离伴脉络膜脱离
retinal detachment - cytokines
- enzyme-linked immunosorbent assay
- choroidal detachment associated with retinal detachment
- 于旭辉
- 葛红岩
- 张蕾
- 宋晗
- 遇颖
- 王宇虹
YU Xuhui1- GE Hongyan1
- ZHANG Lei1
- SONG Han1
- YU Ying1
- WANG Yuhong2(1.Department of Ophthalmology
- The First Clinical College of Harbin Medical University
- Haerbin 150001
- Heilongjiang
- China
- 2.Department of Neurology
- The First Clinical College of Harbin Medical University
- Haerbin 150001
- Heilongjiang
- China)
- 于旭辉
- 葛红岩
- 张蕾
- 宋晗
- 遇颖
- 王宇虹
YU Xuhui1- GE Hongyan1
- ZHANG Lei1
- SONG Han1
- YU Ying1
- WANG Yuhong2(1.Department of Ophthalmology
- The First Clinical College of Harbin Medical University
- Haerbin 150001
- Heilongjiang
- China
- 2.Department of Neurology
- The First Clinical College of Harbin Medical University
- Haerbin 150001
- Heilongjiang
- China)