贵州医科大学学报

2017, v.42;No.202(07) 745-748

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原发性肝癌患者血清代谢产物初步研究
Serum Metabonomic Study of Primary Hepatocellular Carcinoma

蔡坤;田舍;喻超;陈世裕;张乙凡;潘耀振;孙诚谊;
CAI Kun;TIAN She;YU Chao;CHEN Shiyu;ZHANG Yifan;PAN Yaozhen;SUN Chengyi;Department of Hepatic-Biliary-Pancreatic Surgery,the Affiliated Hospital of Guizhou Medical University;Key Laboratory of Hepatobiliary and Pancreatic Surgery,Guizhou Medical University;

摘要(Abstract):

目的:分析原发性肝癌(PHC)患者血清中小分子代谢产物的改变,为早期诊断和治疗原发性肝癌提供依据。方法:收集52例原发性肝癌(实验组)和同期17例正常体健者(对照组)血清,Bruker 600核磁共振检测两组血清中小分子代谢产物,通过主成分分析法比较两组受检者血清中代谢产物含量的差别情况。结果:与对照组比较实验组血清中α-葡萄糖、β-葡萄糖、低密度脂蛋白、极低密度脂蛋白、糖蛋白、乳酸盐、异丁酸盐、丙氨酸、精氨酸、赖氨酸及肌酸等含量明显增加,差异有统计学意义(P<0.05)。结论:质子核磁共振代谢组学技术可以无创而有效地识别肝癌患者血清代谢变化,有望成早期诊断原发性肝癌的一种新的方法。
Objective: To analyze the change of serum metabonomics of primary hepatocellular carcinoma( PHC) patients and provide reference for early diagnosis and treatment. Methods:1H NMR was performed on 52 blood specimens from PHC patient and 17 specimens from healthy controls. Bruker 60 MRI tested small molecule metabolites of both groups,Principal Component Analysis was adopted to analyze the metabolites content differences of both groups. Results: Compared with control group,α-glucose,β-glucoseβ,low density lipoprotein( LDL),very low density lipoprotein( VLDL),glycoprotein,lactate,isobutyrate,alanine,arginine,lysine and creatine of experiment group increased obviously,differences were statistically significant( P < 0. 05). Conclusion: Proton magnetic resonance metabolomic techniques could recognize the serum metabolite changes in PHC patients in noninvasive manner,it should be expected to be established as a new method to diagnose PHC.

关键词(KeyWords): 肝肿瘤;血清;代谢产物学;质子核磁共振;诊断
hepatocellular carcinoma;serum;metabonomic study;proton magnetic resonance;diagnosis

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基金项目(Foundation): 国家自然科学基金资助项目(81560477);; 贵州省科学技术基金[黔科合J字(2015)2013号];; 贵州省卫生计生委科学技术基金项目(gzwjkj2014-1-004,gzwjkj 2014-2-112);; 贵州省第四批人才基地基金资助[黔省专合字(2012)94号];; 贵州省科学技术厅-贵阳医学院院士工作站肝胆外科分站[黔科合院士站(2015)4013];; 贵州省高层次创新人才培养计划“十”层次人才[黔科合平台人才(2016)5647];; 贵州省孙诚谊“肝胆胰脾疾病诊治”研究生导师工作室[黔教研合GZS(2016)09];; 贵州省肝胆外科临床医学研究中心[黔科合平台人才(2017)5404]

作者(Author): 蔡坤;田舍;喻超;陈世裕;张乙凡;潘耀振;孙诚谊;
CAI Kun;TIAN She;YU Chao;CHEN Shiyu;ZHANG Yifan;PAN Yaozhen;SUN Chengyi;Department of Hepatic-Biliary-Pancreatic Surgery,the Affiliated Hospital of Guizhou Medical University;Key Laboratory of Hepatobiliary and Pancreatic Surgery,Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2017.07.001

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