肖业臣;黄亚东;张祥胜;李校堃;
XIAO Yechen1,HUANG Yadong2,ZHANG Xiangsheng3,LI Xiaokun1,4(1.Engineering Research Center of Bioreactor and Pharmaceutical Development,Ministry of Education,Jilin Agricultural University,Changchun 130118,Jilin,China;2.The Biopharmaceutical R&D Center of Jinan University,Guangzhou 510642,Guangdong,China;3.College of Life Science,Yangtze University,Jingzhou 434025,Hubei,China;4.Department of Pharmacology,Wenzhou Medical College,Wenzhou 325027,Zhejiang,China)

• 1:吉林农业大学生物反应器与药物开发教育部工程研究中心
• 2:暨南大学医药生物技术研究开发中心
• 3:长江大学生命科学学院
• 4:吉林农业大学生物反应器与药物开发教育部工程研究中心 吉林长春130118

摘要(Abstract)：

目的:研究CNE2细胞中Smad4基因的突变。方法:运用RT-PCR方法从CNE2细胞中克隆人Smad4cDNA序列,然后与正常鼻咽组织及GenBank序列进行比较分析。结果:CNE2细胞中Smad4基因没有发生任何形式的突变。结论:CNE2细胞可能是一种非Smad4突变的恶性肿瘤,构建的含有人Smad4编码序列的重组质粒,为今后开展Smad4基因的功能研究奠定了基础。
Objective:To investigate the mutation of Smad 4 gene in CNE2 cell line.Methods:Human Smad 4 gene cDNA sequence was obtained from CNE2 cell by using RT-PCR technique,and compared with Smad 4 gene sequences obtained from normal nasopharynx tissue and GenBank.Results:No Smad 4 gene mutation was found in CNE2 cells.Conclusions:CNE2 cell is a kind of malignant tumor without mutation of Smad 4 gene.So,it is suggested that Smad 4 gene may not be possibly a suspectable factor for human nasopharyngeal carcinomas.The recombinant plasmid containing Smad 4 gene developed in this study lays a base for the functional research of Smad 4.

关键词(KeyWords)： 转化生长因子;Smad4基因;癌;鼻咽肿瘤;CNE2细胞;DNA突变分析
transforming growth factors;genes,Smad 4;carcinoma;nasopharyngeal neoplasms;CNE2 cell line;DNA mutational analysis

Abstract:

Keywords:

基金项目(Foundation): 浙江省卫生高层次创新人才培养工程资助项目

作者(Author): 肖业臣;黄亚东;张祥胜;李校堃;
XIAO Yechen1,HUANG Yadong2,ZHANG Xiangsheng3,LI Xiaokun1,4(1.Engineering Research Center of Bioreactor and Pharmaceutical Development,Ministry of Education,Jilin Agricultural University,Changchun 130118,Jilin,China;2.The Biopharmaceutical R&D Center of Jinan University,Guangzhou 510642,Guangdong,China;3.College of Life Science,Yangtze University,Jingzhou 434025,Hubei,China;4.Department of Pharmacology,Wenzhou Medical College,Wenzhou 325027,Zhejiang,China)

Email:

DOI: 10.19367/j.cnki.1000-2707.2008.02.012

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