贵州医科大学学报

2016, v.41;No.192(09) 1029-1032

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扁蒴藤素对紫杉醇耐药乳腺癌细胞生长的抑制作用
Inhibitory Effect of Pristimerin on Growth of Taxol-Resistant Breast Cancer Cells

谢闺娥;杜晶春;董桂兰;徐霞;
XIE Guie;DU Jingchun;DONG Guilan;XU Xia;Kingmed School of Laboratory Medicine,Guangzhou Medical University;Outpatient Department,Guangzhou University;

摘要(Abstract):

目的:研究扁蒴藤素对紫杉醇耐药乳腺癌细胞MCF-7/TAX生长的作用。方法:以乳腺癌细胞株MCF-7为对照,同时制作紫杉醇耐药乳腺癌细胞MCF-7/TAX;在两组细胞中分别给与7种浓度(0.25、0.5、1、2.5、5、10和20μmol/L)扁蒴藤素或紫杉醇培养48 h,用MTT法检测药物对MCF-7和MCF-7/TAX细胞作用的半数抑制浓度(IC50)值,并计算MCF-7/TAX细胞对紫杉醇或扁蒴藤素的耐药指数(RI);同时用Annexin VFITC/PI染色观察扁蒴藤素作用后MCF-7/TAX凋亡细胞比例。结果:MCF-7/TAX对紫杉醇RI达19.39,而扁蒴藤素对MCF-7/TAX细胞的生长有高效抑制作用,且呈现明显的剂量依赖性,对其作用48 h的IC50为0.52μmol/L,MCF-7/TAX对扁蒴藤素的RI仅为0.88;扁蒴藤素作用后,Annexin V染色阳性的MCF-7/TAX细胞比例较对照明显升高。结论:扁蒴藤素能抵抗紫杉醇耐药乳腺癌细胞的耐药性,激活MCF-7/TAX细胞凋亡,对其生长发挥高效抑制作用。
Objective: To investigate the effect of pristimerin on the growth of taxol-resistant breast cancer cells MCF-7 / TAX. Methods: The breast cancer cell line MCF-7 was the control group,and the taxol-resistant breast cancer cells line MCF-7 / TAX was the observation group. In two groups of cells were cultured in 7 kinds of concentrations( 0. 25,0. 5,1,2. 5,5,10 and 20 mol / L) pristimerin and paclitaxel for 48 h. MTT assay was used to detect half inhibition concentration( IC50) value of drug on MCF-7 and MCF-7 / tax cells,and resistance index( RI) of cells to paclitaxel or pristimerin were calculated. At the same time Annexin V-FITC / PI staining was adopted to observe changes of proportion of apoptotic cells after pristimerin involvement. Results: The resistance index of MCF-7 / TAX cells to paclitaxel was 19. 39. After treatment with pristimerin for 48 h,MCF-7 / TAX cells displayed markedly growth inhibition in a dose-dependent manner,and IC50 for 48 h was 0. 52 μM. The resistance index of MCF-7 / TAX cells to pristimerin was 0. 88. After treatment with pristimerin,the proportion of positive MCF-7 / TAX cells of Annexin V-FITC / PI staining was significantly higher than that of the control group. Conclusion: Pristimerin can resist resistance to paclitaxel resistance to breast cancer cells,activate apoptosis of MCF-7 / TAX cells,thus effectively inhibit cancer cells growth.

关键词(KeyWords): 紫杉醇耐药;扁蒴藤素;MCF-7/TAX;凋亡
taxol resistance;pristimerin;MCF-7/TAX;apoptosis

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基金项目(Foundation): 国家自然科学基金(81101682);; 广东省公益研究与能力建设专项资金(2014A020212015)

作者(Author): 谢闺娥;杜晶春;董桂兰;徐霞;
XIE Guie;DU Jingchun;DONG Guilan;XU Xia;Kingmed School of Laboratory Medicine,Guangzhou Medical University;Outpatient Department,Guangzhou University;

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