贵州医科大学学报

2018, v.43;No.211(04) 458-461

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复发性自然流产孕妇血管内皮生长因子与可溶性受体的表达
Expression and Clinical Significance of Vascular Endothelial Growth Factor and Sflt-1 In Patients with Recurrent Spontaneous Abortion

刘明娟;刘谦;李岚;邱秀芳;
LIU Mingjuan;LIU Qian;LI Lan;QIU Xiufang;Department of Obstetrics and Gynecology,People's Hospital of Wuhan University,People's Hospital of Hubei Province;Department of Obstetrics and Gynecology,Fifth Hospital of Wuhan city;

摘要(Abstract):

目的:探讨复发性自然流产(RSA)孕妇血管内皮生长因子(VEGF)与可溶性受体(sflt-1)的表达。方法:73例RSA孕妇为观察组,同期要求行人工流产的正常孕妇50例为对照组,采用酶联免疫法法测定2组孕妇血清VEGF和sflt-1水平,免疫组织化学染色法观察绒毛组织中的VEGF和sflt-1的表达。结果:观察组孕妇血清VEGF与sflt-1表达水平均显著高于对照组,差异具有统计学意义(P<0.05);观察组孕妇的VEGF与sflt-1染色深度、阳性表达率均显著高于对照组,差异具有统计学意义(P<0.05)。结论:VEGF和sflt-1在血清和绒毛组织中的高表达可能是导致RSA胚胎发育障碍的原因之一。
Objective: To investigate the expression and its clinical significance of vascular epithelial growth factor( VEGF) and soluble receptor( sflt-1) in patients with recurrent spontaneous abortion.Methods: 73 patients with recurrent spontaneous abortion as the observation group,50 women with the same period of pregnancy who asked for induced abortion were selected as the control group. The levels of VEGF and sflt-1 in serum were detected by enzyme-linked immunosorbent assay( ELISA). The expressions of VEGF and sflt-1 in villous tissue were observed by immunohistochemical staining.Results: The serum levels of VEGF and sflt-1 in the observation group were significantly higher than that of control group,the differences were statistically significant( P < 0. 05). The dyeing depth of VEGF and sflt-1 was significantly higher than that of control group,positive expression rate was significantly higher than control group,the difference was statistically significant( P < 0. 05). Conclusions:The high expression of VEGF and sflt-1 in serum and villous tissue may be an important cause of embryonic development disorder in RSA patients.

关键词(KeyWords): 流产,自然;复发;绒毛膜绒毛;血管内皮生长因子;可溶性受体;血清;免疫组织化学染色法
abortion,spontaneous;chorionic villus;vascular endothelial growth factor;soluble receptor;serum;immunohistochemical staining method

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基金项目(Foundation): 湖北省卫生厅科研基金资助项目(JX6C-25)

作者(Author): 刘明娟;刘谦;李岚;邱秀芳;
LIU Mingjuan;LIU Qian;LI Lan;QIU Xiufang;Department of Obstetrics and Gynecology,People's Hospital of Wuhan University,People's Hospital of Hubei Province;Department of Obstetrics and Gynecology,Fifth Hospital of Wuhan city;

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DOI: 10.19367/j.cnki.1000-2707.2018.04.018

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