贵州医科大学学报

2016, v.41;No.188(05) 535-538

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钛表面构建成骨细胞膜片的体外研究
Study in vitro of Osteoblast Cell Sheet on Titanium Surface

毛久凤;夏茜;吴镭;杨红;周成菊;方艺;董强;
MAO Jiufeng;XIA Qian;WU Lei;YANG Hong;ZHOU Chengju;FANG Yi;DONG Qiang;Guizhou Medical University;Department of Oral Medicine,the Affiliated Stomatological Hospital of Guizhou Medical University;Guiyang Stomatological Hospital;Department of Pathology,Guizhou Medical University;Department of Prosthodontics,Stomatological College of Guizhou Medical University;

摘要(Abstract):

目的:研究成骨细胞膜片在钛表面的生长情况。方法:将体外培养鉴定的的大鼠成骨细胞接种在纯钛表面,构建大鼠成骨细胞膜片,连续培养1、2、3周,分别于第1、2、3周末收集成骨细胞膜片,倒置显微镜下观察细胞膜片的组成,光镜测量各时间点细胞膜片的厚度。结果:原代培养细胞符合成骨细胞形态学特征;第2、3周末细胞膜片厚度较第1周末细胞膜片厚度明显增加(P<0.05);与第3周末细胞膜片相比,第2周末细胞膜片厚度增加最明显,差异具有统计学意义(P<0.05)。结论:钛表面构建的成骨细胞膜片拥有良好的增殖生长活性。
Objective: To investigate the growth activity of osteoblast cell sheet on the titanium surface. Methods: Rats' osteoblasts were cultured in vitro and identified. Then osteoblast cell sheets were vaccinated on the titanium surfaces and cultured for 1 week,2 weeks and 3 weeks. At different designated time points the cell-sheets were collected with a cell scraper and its structure and composition were examined under the inverted microscope,and the thickness of the cell sheet was measured by light microscope. Results: The cells on cell sheet were consistent with the morphological characteristics of osteoblasts,suggesting the cell sheets were successfully constructed on the titanium surface. The results showed that the thickness of the cell sheet significantly increased at 2~(nd)week and 3~(rd)week after culture compared with at the 1~(st) week( P < 0. 05). The thickness of the cell sheet increased more significantly at the 2~(nd)week compared with at the 3~(rd)week( P < 0. 05). Conclusion: The osteoblast cell sheets on the titanium surface have a good proliferation activity.

关键词(KeyWords): 成骨细胞;钛;组织工程;细胞膜片
osteoblast;titanium;tissue engineering;cell sheet

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基金项目(Foundation): 贵州省科技厅省校合作协议项目[黔科合LH字(2014)7115号];; 贵阳市科技计划项目[筑科合同(20141001)39号]

作者(Author): 毛久凤;夏茜;吴镭;杨红;周成菊;方艺;董强;
MAO Jiufeng;XIA Qian;WU Lei;YANG Hong;ZHOU Chengju;FANG Yi;DONG Qiang;Guizhou Medical University;Department of Oral Medicine,the Affiliated Stomatological Hospital of Guizhou Medical University;Guiyang Stomatological Hospital;Department of Pathology,Guizhou Medical University;Department of Prosthodontics,Stomatological College of Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2016.05.010

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