贵州医科大学学报

2001, (02) 129-130

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间接分光光度法测定抗坏血酸含量
Determination of Ascorbic Acid by Indirect Spectrophotometry

徐黔江,许乾丽
XU Qian jiang 1,XU Qian li 2 (1. Department of Pharmacy, Guiyang Medical College,Guiyang 550004, China; 2.The Center of Physic Chemistry Test ,Guiyang 550002, China)

摘要(Abstract):

目的 :测定药物中的抗坏血酸含量。方法 :利用抗坏血酸对铁 (Ⅲ ) - 4- (2 -苯并噻唑偶氮 )邻苯二酚 -乳化剂OP络合物的还原作用 ,生成铁 (Ⅱ ) - 4- (2 -苯并噻唑偶氮 )邻苯二酚 -乳化剂OP络合物 ,使用分光光度法间接测定抗坏血酸。结果 :测定了抗坏血酸片和Vc注射液的含量 ,并与碘量法比较 ,同时还测定了标准回收率。结论 :此法方便快捷 ,测定含量与碘量法一致 ,结果可靠
Objective:To analyse the content of ascorbic acid in medicine.Methods:Ascorbic acid was determined by spectrophotometric method based on the formation of iron(Ⅱ)-4-(2-benzothiazolylazo) pyrocatechol-OP complex which was the product of the redox reaction between ascorbic acid and iron (Ⅲ)-4-(2-benzothiazolylazo) pyrocatechol-OP complex. The proposed method is rapid and satisfactory.Results:The content of Ascorbic Acid in medicines have been determined and compared with the results of iodine method.Conclution:The proposed method is rapid and satisfaction and the results is consistence with the iodine method.

关键词(KeyWords): 抗坏血酸;分光光度法;铁(Ⅲ)-4-(2-苯并噻唑偶氮)邻苯二酚
ascorbic acid; spectrophotometry; iron(Ⅲ)-4-(2-benzothiazolylazo)pyrocatechol

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作者(Author): 徐黔江,许乾丽
XU Qian jiang 1,XU Qian li 2 (1. Department of Pharmacy, Guiyang Medical College,Guiyang 550004, China; 2.The Center of Physic Chemistry Test ,Guiyang 550002, China)

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