贵州医科大学学报

2016, v.41;No.194(11) 1241-1244+1248

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兴义维蚋蚋卵(胚胎)原代培养细胞核型分析
Karyotype Analysis of Primary Cells Cultured from Blackfly Eggs(Embryos) of Simulium(Wilhelmia) xingyiense

周静;刘占钰;徐旭;崔立秋;陈汉彬;杨明;
ZHOU Jing;LIU Zhanyu;XU Xu;CUI Liqiu;CHEN Hanbin;YANG Ming;College of Biology and Engineering,Guizhou Medical University;Clinical Laboratory,the First Affiliated Hospital of Gannan Medical College;Liver Disease Laboratory,the Affiliated Hospital of Guizhou Medical University;College of Basic Medical Sciences,Guizhou Medical University;

摘要(Abstract):

目的:分析兴义维蚋蚋卵(胚胎)原代培养细胞核型。方法:应用组织块法处理野外采集兴义维蚋蚋卵(胚胎),以改良Shields and Sang M3为基础培养基,p H 6.0~6.5,置于28℃生化恒温培养箱中进行原代细胞培养,选取10个处于有丝分裂增殖时期的细胞,通过秋水仙素处理,制作中期染色体标本,进行核型分析。结果:显微镜下观察,前中期染色体为3对(2n=6),染色体凝集程度未达最大化呈现长杆状,染色体长度为9.43~13.58μm;中期染色体为3对(2n=6),高度凝集呈现短杆状,染色体长度为2.43~4.67μm,两条中期染色体臂比值<1.67,属中着丝粒染色体,4条染色体臂比值>1.67,属亚中着丝粒染色体。结论:初步阐析了兴义维蚋蚋卵(胚胎)原代培养细胞核型特征。
Objective: To culture primary cells of blackfly eggs(embryos) and analyze their karyotype. Methods: The tissue block method was applied to treat the blackfly eggs(embryos) from Simulium(Wilhelmia) xingyiense that were collected in field. The blackfly eggs(embryos) were cultured in the modified Schneider's and Sang M3 medium at p H 6. 0 ~ 6. 5,and incubated in a biochemical incubator at 28 ℃. The primary cells in the mitotic and proliferative phase were selected and treated with colchicine,and their metaphase chromosomes were prepared for karyotype analysis. Results: The prometaphase chromosomes are 3 pairs(2n = 6),which were not aggregated at a maximum degree and showed themselves long rhabditiform,and the length of chromosome is 9. 43 ~ 13. 58 μm. Metaphase chromosome was 3 pairs(2n = 6),and which were highly aggregated and showed themselves short rhabditiform,and the length of chromosome was 2. 43 ~ 4. 67 μm. Two metaphase chromosomes' arm ratio is less than 1. 67,belonging tocentric chromosome,and the 4 chromosomes' arm ratio is more than1. 67,belonging to the submetacentric chromosome. Conclusions: The karyotype characteristics of primary cells cultured from the blackfly eggs(embryos) of Simulium(Wi.) xingyiense were preliminarily elucidated in this study.

关键词(KeyWords): 蚋科;兴义维蚋;胚胎;原代细胞;核型分析
Simuliidae;Simulium(Wilhelmia) xingyiense;embryos;primary cells;karyotype analysis

Abstract:

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基金项目(Foundation): 国家自然科学基金(30660021);; 贵州省社会发展科技攻关计划[黔科合(2012)3100];; 贵州省科技计划项目[黔科合LH字(2016)7357];; 贵州医科大学高等学校2015年大学生创新创业训练计划项目(201510660034)

作者(Author): 周静;刘占钰;徐旭;崔立秋;陈汉彬;杨明;
ZHOU Jing;LIU Zhanyu;XU Xu;CUI Liqiu;CHEN Hanbin;YANG Ming;College of Biology and Engineering,Guizhou Medical University;Clinical Laboratory,the First Affiliated Hospital of Gannan Medical College;Liver Disease Laboratory,the Affiliated Hospital of Guizhou Medical University;College of Basic Medical Sciences,Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2016.11.001

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