贵州医科大学学报

2016, v.41;No.191(08) 874-877

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激活星形胶质细胞α7胆碱能受体后内源性Cryab蛋白的表达
Endogenous Cryab Protein Expression after Activation of α7 n AChRs of Astrocytes

任真奎;何婧;吴昌学;官志忠;禹文峰;
REN Zhenkui;HE Jing;WU Changxue;GUAN Zhizhong;YU Wenfeng;Key Lab of Medical Molecular Biology,Guizhou Medical University;

摘要(Abstract):

目的:探讨尼古丁激活SD大鼠星形胶质细胞α7胆碱能受体(α7 n AChRs)后内源性B-晶状体蛋白(Cryab)表达。方法:分离24 h新生乳鼠大脑皮质,原代培养并鉴定为星形胶质细胞后,将培养第3~4代的星形胶质细胞分为正常对照组、尼古丁处理组和α7 n AChRs阻断剂(MLA)联合尼古丁处理组;正常对照组不加尼古丁和MLA,尼古丁处理组以1、5及10μmol/L浓度的尼古丁处理星形胶质细胞6、12、18及24 h,MLA联合尼古丁处理组以0.05、0.1、0.15及0.2μmol/L浓度的MLA预先处理星形胶质细胞2 h后,再加入尼古丁处理组最佳浓度的尼古丁和最佳培养时间培养细胞;运用蛋白印迹(Western blotting)方法检测3组星形胶质细胞内源性Cryab蛋白的表达。结果:与正常对照组比较,尼古丁处理组的星形胶质细胞内源性Cryab蛋白上调(P<0.05);与尼古丁处理组比较,MLA联合尼古丁处理组的星形胶质细胞内源性Cryab蛋白上调受到明显抑制(P<0.01)。结论:尼古丁激活星形胶质细胞α7n AChRs可上调内源性Cryab蛋白表达,该表达可被MLA阻断。
Objective: To investigate the expression of endogenous Cryab protein after the activation of α7 n AChRs by nicotine in SD rat astrocytes. Methods: The rat cerebral cortex of 24 h neonatal was isolated. After primary culture and identification of astrocytes,the astrocytes cultivated for 3 ~ 4 generation were divided into normal control group,nicotine treatment group and α7 n AChRs blocking agent( MLA) plus nicotine treatment group. Normal control group did not receive nicotine and MLA,1,5and 10 μmol / L nicotine was used to treat astrocytes for 6,12,18 and 24 h in nicotine treatment group,and in MLA plus nicotine treatment group 0. 05,0. 1,0. 15 and 0. 2 μmol / L MLA treated astrocytes for 2 h previously and nicotine of optimal concentration was used to cultivate the astrocytes in optimal cultivation time. Western blotting was used to detect the expression of endogenous Cryab protein in astrocytes the three groups. Results: Compared with normal control group,the endogenous Cryab protein expression up-regulated significantly in nicotine treatment group( P < 0. 05). Compared with nicotine treatment group,the up-regulation of endogenous Cryab protein expression in MLA plus nicotine treatment group was significantly inhibited( P < 0. 01). Conclusions: The activation of α7n AChRs of astrocytes by nicotine can up-regulate endogenous Cryab protein expression,which can beblocked by α7 n AChRs blocker MLA.

关键词(KeyWords): 星形胶质细胞;B-晶状体蛋白;α7胆碱能受体;尼古丁;阿尔茨海默氏病
astrocytes;Cryab;α7 cholinergic receptor;nicotine;Alzheimer's disease

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81360199);; 教育部科学技术研究项目(213032A);; 贵州省国际科技合作项目[黔科合外G字(2013)7026号]

作者(Author): 任真奎;何婧;吴昌学;官志忠;禹文峰;
REN Zhenkui;HE Jing;WU Changxue;GUAN Zhizhong;YU Wenfeng;Key Lab of Medical Molecular Biology,Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2016.08.002

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