孟鑫;刘瑶;陈瑞;蔡进;毛政益;刘珈伲;刘丽娜;黄静;
MENG Xin;LIU Yao;CHEN Rui;CAI Jin;MAO Zhengyi;LIU Jiani;LIU Lina;HUANG Jing;College of Pharmacy,Guizhou Medical University;

• 1:贵州医科大学药学院

摘要(Abstract)：

目的:考察大乌泡根总黄酮的提取工艺。方法:选取料液比、乙醇浓度、提取时间、提取温度为考察因素,以总黄酮得率为评价指标,采用正交试验对提取工艺进行优化,并用紫外分光光度法对大乌泡根总黄酮含量进行测定。结果:大乌泡根总黄酮最佳提取工艺参数为料液比为1∶10,乙醇溶液体积分数为60%,提取时间为2 h,提取温度为70℃;该条件下测定大乌泡根总黄酮含量为4.8%。结论:大乌泡根总黄酮含量的测定方法简便,准确可靠,为大乌泡药材质量控制提供基础。
Objective:To explore the extraction process of total flavonoids in the root of Rubus multibracteatus Levl & Vant.Methods:The solid-liquid ratio,ethanol concentration,extraction time,extraction temperature were selected as factors and the yield of total flavonoids as evaluation index.The extraction process was optimized by orthogonal test,and the content of total flavonoids in the root of Rubus multibracteatus Levl & Vant was determined by UV spectrophotometry.Results:The optimum parameters of extraction process of total flavonoids from the root of Rubus multibracteatus Levl & Vant was a follow:solid-liquid ratio 1∶10,ethanol volume fraction 60%,extraction time 2 h,extraction temperature 70 ℃.Under above condition,the content of total flavonoids from the root of Rubus multibracteatus Levl & Vant was 4.8%.Conclusion:The method is practical,accurate and reliable,and can be used as quality control method of Rubus multibracteatus Levl & Vant.

关键词(KeyWords)： 中草药;植物提取物;大乌泡;总黄酮;正交试验
drug;Chinese herbal;plants extracts;Rubus multibracteatus Levl & Vant;total flavonoids;orthogonal experiment

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅中药攻关项目[黔科合ZY(2011)3005]

作者(Author): 孟鑫;刘瑶;陈瑞;蔡进;毛政益;刘珈伲;刘丽娜;黄静;
MENG Xin;LIU Yao;CHEN Rui;CAI Jin;MAO Zhengyi;LIU Jiani;LIU Lina;HUANG Jing;College of Pharmacy,Guizhou Medical University;

Email:

DOI: 10.19367/j.cnki.1000-2707.2016.12.010

参考文献(References)：

• [1]邱德文,杜江.中华本草苗药卷[M].贵阳:贵州科技出版社,2005:62.
• [2]贵州省药品监督管理局编委.贵州省中药材、民族药材质量标准[M].贵阳:贵州科技出版社,2003:30.
• [3]汪毅,司晓文,夏同珩,等.中国苗族药物彩色图集[M].新疆:新疆科学技术出版社,2003:57.
• [4]徐树芸.贵州十种民族药的应用研究[J].世界科学技术-中医药现代化,2006(6):73-78.
• [5]程伟,秦文杰,陈索红,等.大乌泡叶中总黄酮含量测定[J].中国中医药信息杂志,2009(9):42-43.
• [6]李天平,张彦民,李淼,等.大乌泡抗炎活性成分的筛选及GC/MS分析[N].2009年药物分析论文汇总,2009:221-225.
• [7]熊山.中药楮实子及大乌泡的化学成分研究[D].贵州:贵阳中医学院,2009:1-3.
• [8]聂彝,余陈欢,王芳芳,等.石荠芋总黄酮抗炎作用及其机制研究[J].时珍国医国药,2008(1):65-66.
• [9]宣以巍,刘英,江萍,等.柑橘类黄酮的生理活性及其研究现状[J].科技通报,2008(4):494-497.
• [10]陈丛瑾,王琪,李欣.黄酮类化合物抗氧化和抑菌生物活性研究进展[J].中国药房,2011(35):3346-3348.
• [11]刘文丽,余鲁海,马燕.紫外分光光度法在黄酮类化合物含量测定中的应用[J].新疆中医药,2006(3):85-86.
• [12]王向军.两头毛中总黄酮提取工艺[J].中国实验方剂学杂志,2011(13):50-52.
• [13]陈琦.正交试验优化羊蹄总黄酮提取工艺[J].中国医药指南,2010(25):12-13.
• [14]徐保利,管慧洁,王冰,等.棉灯笼果实总黄酮提取工艺[J].中国实验方剂学杂志,2011(21):33-35.

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