刘瑶;陈瑞;蔡进;刘珈伲;刘丽娜;黄静;
LIU Yao;CHEN Rui;CAI Jin;LIU Jiani;LIU Lina;HUANG Jing;College of Pharmacy,Guizhou Medical University;

• 1:贵州医科大学药学院

摘要(Abstract)：

目的:建立大乌泡药材的薄层色谱鉴别方法。方法:以槲皮素和山柰酚为对照品,甲苯-乙酸乙酯-甲酸(5∶4∶1,V/V/V)为展开剂,定量吸取对照品溶液、供试品溶液,分别点于同一块硅胶G薄层板上,1%Al Cl_3及乙醇溶液显色后,置于紫光灯下365 nm处检视。结果:27批大乌泡药材薄层色谱中,在与对照品色谱相应位置上显相同颜色的荧光斑点。结论:薄层色谱方法可用于大乌泡药材的质量控制。
Objective:To establish the TLC identification method of Rubus multibracteatus Levl &Vant.Methods:Quercetin and kaempferol was used as the reference substance.The spread solvent was made of toluene-ethyl acetate-formic acid(5:4:1,V/V/V).The quantitative reference substance solution and test solution were drawn onto the same silica gel G.Drawing quantitative reference substance solution and test solution on a silica gel G.Color display was made by spraying 1% aluminum chloride acid ethanol.The fluorescence chromatogram was detected at 365 nm in the UV lamp.Result:The spots in the chromatogram of the 27 batches of Rubus Multibracteatus Levl & Vant had the same color as quercetin and kaempferol in corresponding position.Conclusion:Thin layer chromatography method can be used for the quality control of Rubus multibracteatus Levl & Vant.

关键词(KeyWords)： 中草药;色谱法,薄层;大乌泡;山柰酚;槲皮素
drug;Chinese herbal;chromatography;thin layer;Rubus multibracteatus Levl & Vant;kaempferol;quercetin

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅中药攻关项目[黔科合ZY(2011)3005]

作者(Author): 刘瑶;陈瑞;蔡进;刘珈伲;刘丽娜;黄静;
LIU Yao;CHEN Rui;CAI Jin;LIU Jiani;LIU Lina;HUANG Jing;College of Pharmacy,Guizhou Medical University;

Email:

DOI: 10.19367/j.cnki.1000-2707.2016.12.009

参考文献(References)：

• [1]贵州省药品监督管理局.贵州省中药材、民族药材质量标准[M].贵阳:贵州科技出版社,2003:30.
• [2]孟鑫.大乌泡镇痛、抗炎药效物质基础研究[D].贵阳:贵州医科大学,2015.
• [3]国家药典委员会.中华人民共和国药典(一部)[S].2010年版.北京:中国医药科技出版社,2010:附录34.
• [4]王岩,宋良科,董关涛,等.尖瓣过路黄中槲皮素和山柰酚的定性定量分析[J].现代中药研究与实践,2011(3):25-27.
• [5]程煜凤,王丹,李莉.金雀花的薄层鉴别及黄酮类化合物的含量测定[J].新疆医科大学学报,2013(8):1094-1097.
• [6]冼寒梅,陈勇,唐林.白子菜药材薄层色谱鉴别的实验研究[J].辽宁中医杂志,2008(2):259-260.
• [7]王祥培,许士娜,吴红梅,等.天胡荽药材中槲皮素的薄层色谱鉴别与含量测定[J].时珍国医国药,2010(1):247-249.
• [8]王焕芸,王玉华,贾彦胜,等.丁香药材中槲皮素的定性鉴别和含量测定[J].内蒙古医学院学报,2008(6):534-537.
• [9]李晓娟,薛培凤,鞠爱华,等.蒙药玉簪花中山柰酚的HPLC含量测定与薄层色谱鉴别[J].内蒙古医学院学报,2011(4):307-310.
• [10]李春红,潘宏,何兵,等.香椿嫩叶中槲皮素和山柰酚的同板薄层鉴别及总黄酮含量测定[J].泸州医学院学报,2010(4):247-249.
• [11]赵东升,王强,王波,等.三七止血片薄层色谱鉴别及其有效成分槲皮素含量测定[J].沈阳部队医药,2008(4):237-239.
• [12]张玲,王德群,查日维,等.覆盆子中山柰酚的TLC鉴别和HPLC测定[J].中国现代中药,2012(2):12-14.

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