贵州医科大学学报

2018, v.43;No.216(09) 1055-1059

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鼻咽癌患者肿瘤组织及血清长链非编码RNA BC200的表达
Expression of Long Non-coding RNA BC200 in Tumor Tissues and Serum of Patients with Nasopharyngeal Carcinoma

黄丽;李宝瑞;
HUANG Li;LI Baorui;The Fourth People's Hospital of Guangyuan;

摘要(Abstract):

目的:探究鼻咽癌(NPC)患者肿瘤组织及血清中长链非编码RNA BC200的表达水平及其临床意义。方法:收集74例NPC患者肿瘤组织标本及相应癌旁组织标本、患者入院时的血清样本及同期体检中心50例正常人群血清样本,采用实时荧光定量PCR(RT-qPCR)技术检测患者NPC组织与相应癌旁组织标本BC200表达,检测NPC患者血清与正常人群血清样本中BC200表达的差异,分析NPC患者肿瘤组织标本与TNM分期、血清样本BC200表达与肿瘤淋巴结转移及总生存时间的关系。结果:NPC患者肿瘤组织BC200的表达显著高于相应癌旁组织,血清中BC200的表达显著高于正常人群,差异有统计学意义(P <0. 05); NPC患者肿瘤组织中BC200高表达与患者TNM分期有关,血清中BC200高表达与患者淋巴结转移有关,差异有统计学意义(P <0. 05); NPC患者肿瘤组织及血清BC200高表达的患者总生存率均显著低于低表达患者,差异有统计学意义(P<0. 05)。结论:NPC患者肿瘤组织及血清中均存在BC200的高表达,且其表达水平与患者病情进展及总生存率密切相关,可能是NPC促癌基因。
Objective: To investigate the expression level of long non-coding RNA BC200( BC200)in tumor tissues and serum of patients with nasopharyngeal carcinoma( NPC) and to reveal its potential clinical significance. Methods: The tumor tissues and serum samples of 74 cases with NPC and corresponding paracancerous tissues collected from July 2012 to September 2014 in Department of E. N. T.in our hospital and the serum samples of 50 normal controls collected from Medical Examination Center were compared and analyzed. Real-time quantitative PCR( RT-qPCR) was used to detect the difference of the expression of BC200 between the NPC and paracancerous tissues,and the difference between the serum samples of patients with NPC and the normal group. The correlation of the expression of BC200 in NPC tissues and patients' serum samples and the clinical data and prognosis of the patients was analyzed. Results: The expression of BC200 in NPC tissues was significantly higher than that in paracancerous tissues,while the expression of BC200 in patients' serum samples was significantly higher than that in the normal group,and the difference was statistically significant( P < 0. 05). The expression of BC200 in tumor tissues was significantly correlated with TNM staging( P < 0. 05),and the serum was significantly correlated with lymph node metastasis. The overall survival rate of patients with high expression of BC200 in tumor tissues and serum was significantly lower than that in patients with low expression,and the difference was statistically significant( P < 0. 05). Conclusion: Tumor tissues and serum of patients with NPC possess high expression of BC200 and such expression is closely related to the progression and prognosis of patients. It is a potential promoter of NPC.

关键词(KeyWords): 鼻咽肿瘤;肿瘤转移;肿瘤分期;生存时间;长链非编码
nasopharyngeal carcinoma;neoplasm metastasis;neoplasm staging;survival time;long non-coding RNA BC200

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作者(Author): 黄丽;李宝瑞;
HUANG Li;LI Baorui;The Fourth People's Hospital of Guangyuan;

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