贵州医科大学学报

2016, v.41;No.195(12) 1419-1423+1429

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

氯离子通道蛋白-3对胶质瘤细胞增殖和侵袭的影响
The Molecular Mechanism of ClC-3 in the Proliferation and Invasion of Malignant Glioma Cells

张广宇;贾世峰;曹旭华;张晓炜;焦保华;
ZHANG Guangyu;JIA Shifeng;CAO Xuhua;ZHANG Xiaowei;JIAO Baohua;Department of Neurology,Second Hospital of Hebei Medical University;Department of Oncology,Affiliated Hospital of North China University of Science and Technology;

摘要(Abstract):

目的:观察氯离子通道蛋白-3(ClC-3)对胶质瘤BT-235细胞增殖和侵袭的影响的子机制。方法:采用siRNA技术构建ClC-3表达沉默的人胶质瘤BT-235细胞株,设为siClC-3组,并设置空白对照组和空载体组;采用Western bolting检测3组细胞中ClC-3的表达,CCK8法检测细胞增值活性,ranswell实验用于测定细胞侵袭能力,通过测量细胞容积检测细胞调控性容积下降率,流式细胞术用于测定细胞周期;采用Western bolting检测cyclin D1、MMP-2和MMP-9的表达。结果:相对于空白对照组,siClC-3组ClC-3表达明显降低(P<0.01),细胞增殖活性明显降低(P<0.01),细胞迁移率明显降低(P<0.01);空白对照组细胞调控性容积下降率为(79.35±2.31)%,而siClC组仅为(19.46±1.76)%,差异有统计学意义(P<0.01);培养48 h的空白对照组BT-235细胞G0/G1期占比率为(57.2±3.1)%,而siClC-3组G0/G1期占比率提升至(71.3±4.0)%,产生明显的G0/G1期阻滞,差异有统计学意义(P<0.01);相对于空白对照组,siClC-3组G1期调控蛋白cyclin D1、侵袭相关蛋白MMP-2和MMP-9表达水平明显降低(P<0.01)。结论:ClC-3能明显增强胶质瘤BT-235细胞增殖和侵袭能力,其作用机制与增加细胞调控性容积下降率,诱导cyclin D1、MMP-2和MMP-9表达有关。
Objective:To observe the effects of ClC-3 on the proliferation and invasion of malignant glioma cells,and to explore its molecular mechanism.Methods:ClC-3 knockdown human glioma BT-235 cell line was constructed by siRNA,and assigned as si CIC-3 group,blank control group and empty vector group were also established;Western blotting was used to detect the expressions of ClC-3;CCK8 method was used to detect the cell proliferation activity;transwell method was used to detect invasive ability;the measure of cell volume was used to detect the decrease of regulatory volume;flow cytometry was used to detected cell cycle;Western blotting was used to detect the expressions of cyclin D1,MMP-2 and MMP-9.Results:Compared with the blank control group,the expression of ClC-3 in siClC-3 group was significantly decreased(P<0.01),the cell proliferation activity was significantly decreased(P<0.01),the cell migration rate was decreased(P<0.01).The regulatory volume decrease rate in normal control group were(79.35 ± 2.31) %,while in siClC-3 group were(19.46 ±1.76) %,the difference was statistically significant(P<0.01).The percentage of G0/G1 phase in the blank control group were(57.2 ± 3.1) %,but siClC-3 group increased into(71.3±4.0) %,the difference was statistically significant(P<0.01).Compared with the blank control group,the expression of cyclin D1,MMP-2 and MMP-9 were significantly decreased in siClC-3group(P<0.01).Conclusion:ClC-3 could significantly enhance the proliferation and invasion of glioma BT-235 cells,and the underlying mechanism is related to the increasing of cell-regulated volume decrease rate and the up-regulation of cyclin D1,MMP-2 and MMP-9.

关键词(KeyWords): 氯离子通道蛋白-3;神经胶质瘤;增殖;侵袭
chloride channel-3;glioma;proliferation;invasion

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 张广宇;贾世峰;曹旭华;张晓炜;焦保华;
ZHANG Guangyu;JIA Shifeng;CAO Xuhua;ZHANG Xiaowei;JIAO Baohua;Department of Neurology,Second Hospital of Hebei Medical University;Department of Oncology,Affiliated Hospital of North China University of Science and Technology;

Email:

DOI: 10.19367/j.cnki.1000-2707.2016.12.012

参考文献(References):

文章评论(Comment):

序号(No.) 时间(Time) 反馈人(User) 邮箱(Email) 标题(Title) 内容(Content)
反馈人(User) 邮箱地址(Email)
反馈标题(Title)
反馈内容(Content)
扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享