贵州医科大学学报

2010, v.35;No.142(01) 47-49

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大肠癌患者T细胞亚群和NK细胞活性检测的意义
Determination of T Cell Subgroups and NK Cells in Peripheral Blood of Colon Cancer Patients and Its Clinical Significance

姬清华;李国胜;张汝一;颜登国;
JI Qinghua,LI Guosheng,ZHANG Ruyi,YAN Dengguo(Coloproctological Department,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,Guizhou,China)

摘要(Abstract):

目的:通过检测大肠癌患者外周血T淋巴细胞亚群及NK细胞活性的表达,研究大肠癌患者免疫状况与肿瘤分期的关系,以预测术后恢复情况。方法:采用流式细胞分析法检测60例大肠癌患者和30例对照者外周血中T淋巴细胞亚群及NK细胞活性。结果:大肠癌患者CD4+细胞值、CDM4+/CD8+比值下降且NK细胞活性显著降低,CD8+细胞值显著高于对照组,这种变化随临床分期的增高更加显著(P<0.05);化疗后CD4+细胞值、CD4+/CD8+比值的下降和NK细胞活性的降低及CD8+细胞值的增高更为突出(P<0.01);术后发生转移的患者CD4+细胞值、CD4+/CD8+比值下降及NK细胞活性下降,CD8+细胞值再回升。结论:大肠癌患者细胞免疫功能明显紊乱,并随病情进展及肿瘤转移而加重,切除肿瘤有助于改善患者细胞免疫功能。
Objective:To detect lymphocyte T subsets and NK cells in peripheral blood of patients with colon cancer(CC),and to investigate the relationship between the immune function and Dukes stage of the disease.So as to provide information for prognosis of the patients after operation.Methods:Lymphocyte T subsets and NK cells in peripheral blood of 60 CC patients and 30 healthy people(control group)were detected with flow cytometry.Result:The amounts of T cell CD4+ and the rations of CD4+/CD8+,and NK cytotoxicity were significantly higher,and the amounts of T cell CD8+ were significantly lower in CC patients than those in control group;These changes became more obvious along with the decrease of Dukes stages of the disease(P<0.05).After the chemotherapy,the increase of T cells CD4+,CD4+/CD8+ ratio,and NK cytotoxicity and the increase of T cell CD8+ were getting more remarkable(P<0.01).The same occurred in cases with metastasis after operation.Conclusions:Cell immune function disorder occurs in CC patients,and becomes more severe along with the development of disease and metastasis.Excision of tumor might benefit the improvement of cell immune function of the patients.

关键词(KeyWords): 肠肿瘤;淋巴细胞亚群;杀伤细胞,天然;流式细胞术
intestinal neoplasms;lymphocyte subsets;killer cells,natural;flow cytometry

Abstract:

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作者(Author): 姬清华;李国胜;张汝一;颜登国;
JI Qinghua,LI Guosheng,ZHANG Ruyi,YAN Dengguo(Coloproctological Department,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,Guizhou,China)

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DOI: 10.19367/j.cnki.1000-2707.2010.01.015

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