贵州医科大学学报

2005, (03) 232-233+238

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反相高效液相色谱法测定心脑联通胶囊中大黄素的含量
Determination of Emodin in Xinnaoliantong Capsules with RP-HPLC

丁贤贵,刘丽娜,李勇军,何迅
DING Xian-gui 1, LIU Li-na 2, LI Yong-jun 2, HE Xun 2 (1.Guiyang Office of Shanxi Topsun Pharmacy Co.Ltd, Guiyang 550004, China; 2. School of Pharmacy, Guiyang Medical College, Guiyang 550004, China)

摘要(Abstract):

目的:建立心脑联通胶囊中大黄素的含量测定方法。方法:采用高效液相色谱法测定大黄素的含量。色谱柱:HypersilC18(4.6mm×150mm,5μm);流动相:甲醇-0.2%磷酸水溶液(75∶25);流速:1ml/min;检测波长:438nm。结果:大黄素的线性范围为0.072~1.152μg(γ=0.9999),平均回收率为99.48%,其RSD为2.22%(n=9)。结论:反相高效液相色谱法准确,简便,快速,灵敏度高,重现性好,具有实用性,可用于心脑联通胶囊的质量控制。
Objective: To establish a RP-HPLC method for determination of the emodin content in Xinnaoliantong Capsules. Methods: The chromatographic condition: Hypersil column (4.6 mm×150 mm, 5 μm) was used. The mobile phase was methanol-0.2%H3PO4(75∶25),with a flow-rate of 1.0 ml/min.The UV detection wavelength was 438 nm. Results: The calibration curve was linear (γ=0.999 9) in the range of 0.072~1.152 μg for emodin. The average recovery rate was 99.48%, with RSD of 2.22%( n =9). Conclusion: This method is accurate, rapid, sensitive with good repeatability, and is a better new method for quality control of Xinnaoliantong Capsules.

关键词(KeyWords): 色谱法,高压液相;植物制剂;大黄素;心脑联通
chromatography,high pressure liquid; plant preparations; emodin; Xinnaoliantong

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作者(Author): 丁贤贵,刘丽娜,李勇军,何迅
DING Xian-gui 1, LIU Li-na 2, LI Yong-jun 2, HE Xun 2 (1.Guiyang Office of Shanxi Topsun Pharmacy Co.Ltd, Guiyang 550004, China; 2. School of Pharmacy, Guiyang Medical College, Guiyang 550004, China)

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