贵州医科大学学报

2003, (06) 477-479

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p53蛋白在中耳乳突胆脂瘤上皮中的表达及意义
The Expression of Protein p53 and Its Significance in Human Middle Ear Cholesteatoma

蒋科会,叶惠平,陈乾美,林尚泽
JIANG Ke-hui, YE Hui-ping, CHEN Qian-mei, LIN Shang-ze (Department of Otorhinolaryngology, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China)

摘要(Abstract):

目的 :探讨p5 3蛋白在中耳继发性胆脂瘤上皮中的表达 ,分析其在胆脂瘤上皮增生调节中的可能作用。方法 :运用免疫组织化学EnVision染色法和计算机图像分析法 ,检测 32例胆脂瘤上皮和 9例胆脂瘤患者外耳道皮肤中p5 3蛋白的表达情况。结果 :p5 3蛋白表达在胆脂瘤上皮的全层 ,以基底层和棘层为多 ,p5 3蛋白在外耳道皮肤仅有 1例微弱表达 ,两种组织p5 3阳性表达的平均灰度值 (x±s,下同 )分别为 138 14 11± 10 7388和14 5 95 4 3± 9 3778,两者差异有显著性 (P <0 0 5 )。结论 :p5 3在胆脂瘤上皮的高表达有可能是导致胆脂瘤上皮细胞具有较强增殖能力的原因之一。
Objective: To evaluate the possible role of protein p53 in secondary middle ear cholesteatoma. Methods: The specimens from the secondary middle ear cholesteatoma tissue of 32 cases and the ear canal epithelium of 9 cases were examined with immunohistochemical EnVision method and the data were analyzed with a computer image analyzer. Results: p53-positive cells were observed in all layers of cholesteatoma epithelium. There was weak stain in 1 case of ear canal epithelium. The levels of p53 protein measured in these two types of tissues were 138.141 1±10.738 8 and 145.954 3±9.377 8 (grey degree values) respectively. The difference was significant (P<0.05). Conclusion: The results from this study show that over-expression of p53 in cholesteatoma might cause epithelium hyperproliferation.

关键词(KeyWords): 胆脂瘤,中耳;蛋白质p53;免疫组织化学;增殖
cholesteatoma,middle ear; protein p53; Immunohistochemistry; hyperplasia

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作者(Author): 蒋科会,叶惠平,陈乾美,林尚泽
JIANG Ke-hui, YE Hui-ping, CHEN Qian-mei, LIN Shang-ze (Department of Otorhinolaryngology, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China)

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