贵州医科大学学报

2018, v.43;No.211(04) 373-377+388

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三种不同来源白念珠菌感染对小鼠的致病性研究
Pathogenicity of Three Different Sources of Candida albicans Infection in Mice

夏茂宁;牛雪可;邱文;黄劲;王梅竹;赵亮;廖万清;康颖倩;
XIA Maoning;NIU Xueke;QIU Wen;HUANG Jing;WANG Meizhu;ZHAO Liang;LIAO Wanqing;KANG Yingqian;Department of Microbiology in Guizhou Medical University;Key laboratory of Pathogenic Biology of Guizhou High Education Institution & Key Laboratory in Ministry of Education of Environmental pollution and Disease Surveillance;Department of Biochemistry and Molecular Biology of Guizhou Medical University;Dermatological Department of Annex to Changzheng Hospital Affiliated to Naval military Medical Universit

摘要(Abstract):

目的:探讨3种不同来源白念珠菌对小鼠致病性的差异。方法:选取获得性免疫缺陷综合征(AIDS)、外阴阴道念珠菌病(VVC)患者来源和健康人来源白念珠菌尾静脉注射感染小鼠,同时设立生理盐水尾静脉注射小鼠作为对照组;分别于7~11 d、14~18 d及21~25 d 3个时期段,观察各组小鼠的死亡数及死亡率;无菌分离肾脏靶器官,观察各组小鼠的肾指数及肾脏负荷;收集从肾脏分离得到的白念珠菌,比较3种不同来源白念珠菌株菌丝形成能力。结果:3种不同来源菌株感染的小鼠28 d内死亡率、小鼠肾指数及其肾脏分离菌株菌丝形成能力差异有统计学意义(P<0.05),从高到低依次为AIDS、VVC、健康人来源组;小鼠肾脏负荷组间比较,差异无统计学意义(P>0.05)。结论:菌株来源与其致病性强弱有关,菌株的菌丝形成能力是更为重要的致病因素。
Objective: To study on the pathogenicity of three different sources of Candida albicans infection in mice. Method: The mice infected with acquired immunodeficiency syndrome( AIDS),vulvovaginal candidiasis( VVC) patients and healthy persons were selected,and the mice were injected with normal saline as the control group. The death rate and mortality of mice in each group were observed at 7 ~ 11 d,14 ~ 18 d and 21 ~ 25 d respectively. The kidney target organs were isolated by asepsis,and the renal index and kidney load were observed in each group of mice. To collect Candida albicans isolated from kidney,the hyphal formation ability of three strains of Candida albicans from different sources was compared. Results: There were significant differences in mortality,kidney index and hyphal formation ability of three strains infected with different strains in 28 days( P < 0. 05). The order from high to low is AIDS,VVC and the source group of healthy people. There was no significant difference with comparison of renal load groups in mice( P > 0. 05). Conclutions: The mycelium formation ability of the strain is a more important pathogenic factor because of its strong or weak pathogenicity.

关键词(KeyWords): 白念珠菌;小鼠;模型,动物;肾指数;肾脏负荷;菌丝形成
Candida albicans;mice;model,animal;renal index;renal load;formation of hypha

Abstract:

Keywords:

基金项目(Foundation): 国家自然基金项目(31060006,31260029);; 贵州省卫生计生委科学技术基金项目(gzwjkj 2016-1-049);; 贵阳市人民政府-贵州医科大学科学技术联合基金“贵阳市科技局创新团队”项目(GY2015-18)贵阳市人民政府-贵州医科大学科学技术联合基金项目(GY2016-20);; 贵州省优秀青年人才科技培养项目[黔科合平台人才(2017)5639]

作者(Author): 夏茂宁;牛雪可;邱文;黄劲;王梅竹;赵亮;廖万清;康颖倩;
XIA Maoning;NIU Xueke;QIU Wen;HUANG Jing;WANG Meizhu;ZHAO Liang;LIAO Wanqing;KANG Yingqian;Department of Microbiology in Guizhou Medical University;Key laboratory of Pathogenic Biology of Guizhou High Education Institution & Key Laboratory in Ministry of Education of Environmental pollution and Disease Surveillance;Department of Biochemistry and Molecular Biology of Guizhou Medical University;Dermatological Department of Annex to Changzheng Hospital Affiliated to Naval military Medical Universit

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DOI: 10.19367/j.cnki.1000-2707.2018.04.001

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