贵州医科大学学报

2013, v.38;No.165(06) 593-597

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不同白酒对大鼠肝组织及血清透明质酸和Ⅳ型胶原的影响
Pathological Study on Effect of Different Liquor to Rat Liver Tissue,Hyaluronic Acid and Collagen TypeⅣ

李珀;刘俊玲;何常;程明亮;孙江龄;
LI Bo;LIU Junling;HE Chang;CHENG Mingliang;SUN Jiangling;Department of Pathology ,Guiyang Medical College;Department of Pathology,the Affiliated Hospital of Guiyang Medical College;Department of Infectious Diseases, the Affiliated Hospital of Guiyang Medical College;

摘要(Abstract):

目的:探讨饮用不同白酒对大鼠肝脏及中血清透明质酸和Ⅳ型胶原的影响。方法:雄性SD大鼠131只分为对照组、A酒组(高加低剂量组)、B酒组(高加低剂量组),于2、4、6月末各组随机抽取2只大鼠处死,取肝脏观察组织学改变;6月末,用黄曲霉素B1(AFB1)腹腔注射建立体内肝癌短期模型,6周后,处死大鼠,观察大鼠肝脏病理学变化,取血清进行透明质酸(HA)及Ⅳ型胶原(CⅣ)含量检测。结果:(1)在单纯灌酒后,A酒组发生肝纤维化的比例比B酒组低,AFB1造模后肝纤维化、肝硬化并肝癌的发生率A酒组低于B酒组;(2)与对照组相比,A酒组和B酒组纤维结缔组织增生明显(P<0.05);(3)HA、CⅣ在B酒组中的含量比A酒组中的含量高。结论:2种白酒对肝纤维化、肝硬化模型大鼠的血清及肝组织HA和CⅣ的影响不同,引起的肝损伤程度也不同,B酒可增加HA和CⅣ的水平,相对而言,A酒的作用要轻得多。
Objective: To investigate the influence of different liquor on rat liver. Methods: Different doses of A and B liquor were given to 131 male SD rats by lavage. Two rats were randomly selected from each group,and sacrificed at the end of 2,4,and 6 months afterwards respectively. Histological changes in rat livers were observed. After 6 months,some of the experimental rats were injected aflatoxin B1 into abdomen to make short-term model of liver cancer. The model was established in 6 weeks,and rat livers' histopathological changes were observed. Serum hyaluronic acid( HA) and collagen type Ⅳ( C Ⅳ)contents were detected. Results:( 1) In simple lavage experiment,hepatic fibrosis rate in group A was lower than that in group B; After molding with aflatoxin B1,the hepatic fibrosis rate,and rate of hepatocirrhosis accompanied with hepatocellular carcinoma in group A were lower than those in group B;( 2)Compared with normal control group,fiber hyperplasia occurred obviously in group A and group B( P <0. 05);( 3) Contents of HA and CⅣ were higher in group B than in group A. Conclusions: The levels of HA and CⅣ in serum and liver are different in rat fibrosis model treated by AFB1with liquor A and Brespectively,of which liquor B is more serious.

关键词(KeyWords): 酒;肝疾病,洒精性;肝,纤维化;透明质酸;胶原Ⅳ型;疾病模型,动物;大鼠,Sprague-Dawley
liquor;liver diseases,alcoholic;liver,fibrosis;hyaluronic acid;collagen type Ⅳ;disease models,animal;rats,Sprague-Dawley

Abstract:

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基金项目(Foundation): 国家重点基础研究发展计划(973)课题(NO.2011CB512114);; 贵州省科技计划课题(黔科合SZ[2011]3001)

作者(Author): 李珀;刘俊玲;何常;程明亮;孙江龄;
LI Bo;LIU Junling;HE Chang;CHENG Mingliang;SUN Jiangling;Department of Pathology ,Guiyang Medical College;Department of Pathology,the Affiliated Hospital of Guiyang Medical College;Department of Infectious Diseases, the Affiliated Hospital of Guiyang Medical College;

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