李清春;吕心瑞;潘娅;蒋乃昌;
LI Qing-chun,LV Xin-rui,PAN Ya,JIANG Nai-chang(Department of Physiology,Guiyang Medical College,Guiyang 550004,China)

• 1:贵阳医学院生理学教研室
• 2:贵阳医学院生理学教研室
• 3:贵阳医学院生理学教研室
• 4:贵阳医学院生理学教研室 贵州贵阳550004

摘要(Abstract)：

目的:研究银杏内酯和胰岛素联合应用对拟阿尔茨海默病(AD)大鼠学习记忆的影响。方法:采用冈田酸(okdaicacid,OA)海马CA1区多次微量注射建立拟AD模型大鼠。银杏内酯灌胃和胰岛素侧脑室注射。OA末次注射2周后行水迷宫实验检测其学习记忆能力,水迷宫实验结束后引导各组海马神经元单位放电。结果:模型组大鼠定向航行实验从第1天开始平均逃避潜伏期比对照组明显延长,空间探索实验第3象限活动时间明显缩短及穿越站台次数明显减少;海马神经元放电频率明显减少,放电类型发生改变。与模型组比较,银杏内酯组和银杏加胰岛素组(联合组)大鼠平均逃避潜伏期明显减少,第3象限活动时间明显延长及穿越站台次数明显增多;海马神经元放电增多。与银杏内酯组比较,从第4天开始,联合组大鼠平均逃避潜伏期明显减少;第3象限活动时间明显延长及穿越站台次数明显增多;海马神经元放电频率增多;海马神经元放电类型接近正常。结论:联合应用银杏内酯和胰岛素比单用银杏内酯对拟AD大鼠的作用更明显。
Objective:To observe the effect of ginkgolides and insulin on the learning and memorizing abilities of Alzheimer disease-like(AD-like)rats.Methods:AD-like model was established by injecting okadaic acid(OA)into hippocampal CA1 region of rats.Then,the rats were divided randomly into three groups:model group(MG,did not receive treatment),ginkgolide group(GG,received treatment of ginkgolide),and combination group(CG,received treatment of ginkgolide plus insulin).Ginkgolide was given orally and insulin was injected into the lateral ventricle of the brain.The learning and memorizing abilities of rats were assessed with Morris Water Maze behavioral test.The spontaneous discharge in hippocampus of the rats was observed.Results:Compared with normal rats,the model rats average escape latency was prolonged in place navigation test(P<0.05 or P<0.01),and their swimming time in the third quadrant in spatial probe test shortened.The times of platform-traversing decreased after the platform was removed(P<0.01);The discharge frequency of hippocampus neurons reduced significantly(P<0.001),and the forms of discharge were also changed.Compared with rats in MG,the average escape latency was shortened(P<0.05),the swimming time in the third quadrant prolonged,the times of platform-traversing increased,and the discharge frequency of hippocampus neurons increased significantly(P<0.05)in the rats of GG and CG.Compared with rats in GG,the average escape latency was shortened(P<0.05),the swimming time in third quadrant prolonged,the times of platform-traversing increased,and the discharge frequency of hippocampus neurons increased significantly(P<0.05)in rats of CG.Conclusions:Combination of ginkgolides and insulin are more effective than ginkgolides only to Alzheimer's disease in experimental rats.

关键词(KeyWords)： 阿尔茨海默病;模型,动物;胰岛素;二裂银杏;学习障碍;记忆障碍;自发放电
Alzheimer disease;models,animal;insulin;ginkgo biloba;learning disorders;memory disorders;spontaneous discharge

Abstract:

Keywords:

基金项目(Foundation): 贵州省优秀科技教育人才省长基金资助项目[黔科教办(2001)3号]

作者(Author): 李清春;吕心瑞;潘娅;蒋乃昌;
LI Qing-chun,LV Xin-rui,PAN Ya,JIANG Nai-chang(Department of Physiology,Guiyang Medical College,Guiyang 550004,China)

Email:

DOI: 10.19367/j.cnki.1000-2707.2006.05.009

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