贵州医科大学学报

2006, (05) 391-392

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天麻扩增酶切片段长度多态性反应体系的建立
The Establishment of An Amplified Fragment Length Polymorphism Reaction System with Gastrodia elata Blume

常楚瑞;邹佳宁;宋聚先;王晓丽;
CHANG Chu-rui1,ZOU Jia-ning2,SONG Ju-xian2,WANG Xiao-li1(Department of Medical Plant and Pharmacognosy,School of Pharmacy,Guiyang Medical College,Guiyang 550004,China)

摘要(Abstract):

目的:以天麻为试验材料,建立扩增酶切片段长度多态性(AFLP)分析体系。方法:对天麻DNA提取、酶切-连接、预扩增和选择性扩增等试验过程进行研究。结果:得到了清晰的天麻AFLP指纹图谱。结论:该体系具有良好的稳定性和重复性。
Objective:To establishi an amplified fragment length polymorphism(AFLP)reaction system with Gastrodia elata Blume as material.Methods:Genomic DNA of G.elata was isolated and double enzyme digestion with enzymes MseⅠand PstⅠ,adapter-ligation,pre-amplification,and selective amplification were carried out.The experimental conditions of the processes were optimized.Results:The obtained DNA fingerprints of G.elata were clear with high resolution.Conclusion:It showed that this system is stable and the experiments with the system are repeatable.

关键词(KeyWords): 天麻;多态性,限制性片段长度;扩增酶切片段长度多态性
Gastrodia elata;polymorphism,restriction fragment length;amplified fragment length polymorphism

Abstract:

Keywords:

基金项目(Foundation): 国家科技部2004BA721A33;; 黔科合农社字(2001)1125号;; 黔科合中药专字[2003]51号

作者(Author): 常楚瑞;邹佳宁;宋聚先;王晓丽;
CHANG Chu-rui1,ZOU Jia-ning2,SONG Ju-xian2,WANG Xiao-li1(Department of Medical Plant and Pharmacognosy,School of Pharmacy,Guiyang Medical College,Guiyang 550004,China)

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DOI: 10.19367/j.cnki.1000-2707.2006.05.003

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