贵州医科大学学报

2013, v.38;No.165(06) 621-624

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神经肽K物质对小鼠烫伤创面愈合的影响及机制
Effects of Substance K on Mice Wound Healing and Its Mechanism

王晓辉;方勇;倪涛;
WANG Xiaohui;FANG Yong;NI Tao;The Third People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine;

摘要(Abstract):

目的:研究神经肽K物质对小鼠烫伤创面愈合的影响及机制。方法:建立小鼠烫面创伤模型,检测小鼠创面K物质含量;将小鼠分为3组分别对小鼠创面给予K物质、K物质拮抗剂和生理盐水,用药第5、9天后分别进行创面愈合率、组织病理学分析,检测转化生长因子α(TGF-α)、IL-1、IL-6和肿瘤坏死因子-α(TNF-α)浓度,并体外测定K物质对小鼠成纤维细胞L929增殖的影响。结果:在小鼠创伤模型中K物质含量为155 ng/g,对照组为127 ng/g;给予K物质后能够加速小鼠创面的愈合,而给予K物质拮抗剂使小鼠创面愈合减慢;体外细胞实验表明K物质能促进小鼠成纤维细胞的增殖;结论 K物质能加速小鼠创面愈合。
Objective: To study the relation between substance K and mice wound healing. Methods:Mice wound model was established,the concentration of substance K in wound tissue was measured by ELISA; then the model mice were divided into three groups and treated with substance K,substance K antagonist and normal saline respectively. In the 5thand 9thday after treatment,the wound healing rate and TGF-α,IL-1,IL-6,TNF-α levels were detected. At last,the promoted effect of substance K on L929 cells in vitro as well as the inhibition effect of substance K antagonist were measured; Results: The concentration of substance K in wound tissue was 155 ng / g,while in normal tissue was only 127 ng / g;When treated with substance K,the wound healing process was accelerated. On the contrast,when treated with substance K antagonist,the healing process was inhibited; Also,substance K has a positive effect on the proliferation of L929 cells in vitro. Conclusions: Substance K has a positive effect on wound healing.

关键词(KeyWords): K物质;创面愈合;细胞增殖;细胞因子
substance K;wound healing;cell proliferation;cytokines

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作者(Author): 王晓辉;方勇;倪涛;
WANG Xiaohui;FANG Yong;NI Tao;The Third People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine;

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