贵州医科大学学报

2008, No.133(04) 374-376

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N-乙酰半胱氨酸对急性肺损伤大鼠的保护作用
The Protective Effect of N-acetylcysteine on Acute Lung Injury in Rats Induced by Oleic Acid

黄毅;李秀珍;
HUANG Yi1,LI Xiuzhen2 (1.Department of Respiratory,Zunyi Hospital,Zunyi 563002,Guizhou,China;2.Department of Respiratory,The Affiliated Hosipital of Guiyang Medical Collage,Guiyang 550004,Guizhou,China.)

摘要(Abstract):

目的:探讨N-乙酰半胱氨酸(NAC)对油酸(OA)诱导的急性肺损伤(ALI)大鼠的保护作用。方法:成年雄性SD大鼠18只,随机分成对照组、损伤组(ALI组)和N-乙酰半胱氨酸组(NAC组),后两组在注射OA后3 h检测肺湿重与肺干重比(W/D),肺组织匀浆髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)、丙二醛(MDA)及肺组织病理检查。结果:ALI组MPO、MDA明显升高,SOD明显降低,与对照组比较有明显差异(P<0.01),NAC组MPO、MDA低于ALI组,SOD明显升高,P<0.01;ALI组MPO水平与肺组织病理损伤程度成正相关(r=0.793,P<0.01);肺组织病理组织学显示,NAC预处理能明显改善肺组织病理损伤。结论:NAC对ALI大鼠具有保护作用,其保护机制可能与清除氧自由基有关。
Objective: To investigate the protective effect of N-acetylcysteine(NAC) on lung injury in rats induced by oleic acid(OA).Methods: Eighteen male Sprague-Dawley rats were randomly divided into acute lung injury(ALI) group(n=6),N-acetylcysteine(NAC) group(n=6) and control group(n=6),The lung tissues were collected in 3h after OA or saline administration.Lung weight/dry ratio(W/D),the concentrations of lung homogenate myeloperoxidase(MPO),malondiadehyde(MDA),and superoxide dismutase(SOD) were measured.Meanwhile,The pathological changes of lung were examined with light microscope.Results: In ALI group,levels of lung homogenates MPO,MDA were significantly higher and the level of SOD were significantly lower than those in control group and NAC group(All P<0.01).The levels of MPO in rats of ALI group have a significantly positive correlation with lung pathological injury scores(r=0.793,p<0.01).Histopathologic findings showed less lung injury in NAC group than in ALI group.Conclusions: NAC might have effect of alleviating the lung injury induced by oleic acid,of which the mechanism might be associated with free oxygen radical elimination.

关键词(KeyWords): 急性肺损伤;N乙-酰半胱氨酸;氧自由基;过氧化物酶类;超氧化物歧化酶;丙二醛
acute lung injury;N-acetylcysteine;peroxidases;superoxide dismutase;malondialdehyde

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作者(Author): 黄毅;李秀珍;
HUANG Yi1,LI Xiuzhen2 (1.Department of Respiratory,Zunyi Hospital,Zunyi 563002,Guizhou,China;2.Department of Respiratory,The Affiliated Hosipital of Guiyang Medical Collage,Guiyang 550004,Guizhou,China.)

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DOI: 10.19367/j.cnki.1000-2707.2008.04.015

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