陈艳;包怀恩;李建华;姚曙光;
Chen Yan,et al Department of Parasitology, Guiyang Medical College

• 1: 贵阳医学院寄生虫学教研室
• 2:贵阳医学院寄生虫学教研室
• 3:贵阳医学院寄生虫学教研室
• 4:贵州铜仁地区医院病理科

摘要(Abstract)：

用Dot-ELISA检测了30份斯氏肺吸虫抗原皮试阳性的患者血清,其中,斯氏肺吸虫循环抗体(CAb)呈阳性反应的血清有12份,阳性率为40％(12/30),同时检测的80份正常人血清,全部呈阴性反应。检测结果与间接ELISA法的相同。在实验动物血清的检测方面,斯氏肺吸虫免疫的兔血清,效价达1 :6400,而人工感染血吸虫、华枝睾吸虫、弓形虫溶组织内阿米巴的兔血清及正常兔血清均为阴性。实验表明,Dot-ELISA法具有较高的特异性、敏感性,其检测结果可作为临床诊断的可靠依据。
Pagumogonimus skrjabini circulating antibodies have been successfully detected with Dot-ELISA. The positive rate of 30 people with positive reaction on skin test was 40％ (12/30) while all the 80 normal individuals as controls gace negative rearion, The results were similar to that of ELISA. The titer of reference positive serum against P. skrjabini was 1 : 6400 detected by Dot-ELISA. 30 serum samples from rabbits infected with S. japonicum, C, sinensis, E. histolytica and Γ, gondii and serum samples from 12 normal rabbits were te-s ted with Dot-ELISA as well, The results were all negative. Our studies showed that the Dot-ELISA is a sensitive and specific assay, which is relatively simple to conduct, and it might be a useful tool for diagnosing Pagumongonimiasis.

关键词(KeyWords)： 酶联免疫吸附试验;斯氏肺吸虫
enzyme-linked immunosorbent assay; Pagumogonimus skrjabini

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 陈艳;包怀恩;李建华;姚曙光;
Chen Yan,et al Department of Parasitology, Guiyang Medical College

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DOI: 10.19367/j.cnki.1000-2707.1991.03.016

参考文献(References)：

• 1． Hawkes R, et al.A Dot-Immuno binding Assay for monoclonal and their antibodies. Anal Biochemistry 1982; 119: 142--147．
• 2． 余(氵贺),等。临床免疫技术上册.上海:上海科技出版社,1982:439。
• 3． 陈艳,等.用ABC-Dot-ELISA检测兔弓形虫循环抗体的初步研究.贵阳医学院学报 1990;15(4) :257．

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