贵州医科大学学报

2004, (02) 95-98

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白纹伊蚊贵州麻尾株垂直传播登革病毒实验研究
An Experimental Study on Transovarial Transmission of Dengue Virus in Aedes albopictus of Mawei Strain

赵星,左丽,舒莉萍
ZHAO Xing, ZUO Li, SHU Li-ping(Department of Immunology,Guiyang Medical College,Guiyang 550004,China)

摘要(Abstract):

目的 :研究白纹伊蚊贵州麻尾株对 2型登革病毒 (DEN 2 )的经卵传递能力。方法 :用DEN 2NGC株感染白纹伊蚊贵州麻尾株 ,收集亲代及子 1、2、3代蚊虫标本 ,提取亲代及子代蚊体内病毒总RNA ,通过RT PCR扩增蚊体内DEN 2病毒核酸 ,并用限制性核酸内切酶HinfⅠ酶切鉴定。结果 :感染白纹伊蚊贵州麻尾株后 ,子1~ 3代蚊体内均可检测出病毒核酸。结论 :DEN 2能通过白纹伊蚊贵州麻尾株垂直传播 ,至少可以传 3代。
Objective: To Study the transovarial transmission of dengue virus in Aedes albopictus of Mawei strain. Methods: Adult A. albopictus of Mawei strain was orally infected with dengue-2 virus. Adults of parents and F1~F3 generations of A. albopictus mosquitoes were collected. The total RNA was isolated from the samples collected and the envelope gene of DEN-2 was detected with RT-PCR, and identified with HinfI digestion. Results: Virus nucleic acid were detected from F1~F3 generation of the mosquitoes. Conclusion: These results demonstrate that infected A. albopictus is capable of transmitting dengue virus vertically.

关键词(KeyWords): 登革热病毒;伊蚊属;疾病传播,垂直
dengue virus; Aedes; disease transmission,vertical

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目 (39960 0 0 1 )

作者(Author): 赵星,左丽,舒莉萍
ZHAO Xing, ZUO Li, SHU Li-ping(Department of Immunology,Guiyang Medical College,Guiyang 550004,China)

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DOI: 10.19367/j.cnki.1000-2707.2004.02.001

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